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SpyCatcher/SpyTag-Mediated Self-Assembly of a Supramolecular Complex for Improved Biocatalytic Production of Trehalose

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Abstract

Trehalose has been utilized in the food, cosmetic, and pharmaceutical industries due to its ability to stabilize biomolecules. In this study, we developed a strategy of constructing a self-assembling supramolecular multienzyme complex for the production of trehalose using SpyCatcher and SpyTag. Maltooligosyl trehalose synthase (MTS) and maltooligosyl trehalose trehalohydrolase (MTH) were fused to SpyCatcher and its corresponding ligand, SpyTag. The fused MTS-SpyCatcher (MTS-SC) and MTH-SpyTag (MTH-ST) self-assembled into a supramolecular multienzyme complex (MTS-MTH) via the isopeptide bonds formed between SpyCatcher and SpyTag. MTS-MTH showed the faster reaction rate, which reached the peak of trehalose conversion rate 10 h earlier than the free enzymes. The results demonstrate that the multienzyme self-assembly via the protein-peptide interactions of SpyCatcher and SpyTag can increase the catalytic efficiency of sequential reactions.

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ACKNOWLEDGMENTS

This work was supported by the National Natural Science Foundation of China (no. 21606129, no. 31922070) and the Natural Science Fund for Colleges and Universities in Jiangsu Province (no. 17KJB416005).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing TechUniversity, 211816, Nanjing, Jiangsu, China

    X. Shen

  2. School of Pharmaceutical Sciences, Nanjing TechUniversity, 211816, Nanjing, Jiangsu, China

    S. Tang, Q. Xu & H. Huang

  3. College of Food Science and Light Industry, Nanjing TechUniversity, 211816, Nanjing, Jiangsu, China

    L. Jiang

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  1. X. Shen
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  2. S. Tang
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  3. Q. Xu
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  4. H. Huang
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  5. L. Jiang
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Correspondence to S. Tang or L. Jiang.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Shen, X., Tang, S., Xu, Q. et al. SpyCatcher/SpyTag-Mediated Self-Assembly of a Supramolecular Complex for Improved Biocatalytic Production of Trehalose. Appl Biochem Microbiol 55, 596–602 (2019). https://doi.org/10.1134/S0003683819060115

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