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Activity of Neutral Phytase from Obesumbacterium proteus in Recombinant Strains of Yarrowia lipolytica under Cultivation on Low-Grade Vegetable Substrate

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Abstract

The characteristics of recombinant phytase from Obesumbacterium proteus have been studied after its integration into Yarrowia lipolytica with a Po1f (pUV3-Op) plasmid. Phytase activity was observed in Y. lipolytica Po1f transformants in a wide pH range (3.5–7.5) and remained at 80% of the initial value after 3-min heating at 95°C, which made it possible to add the enzyme to a fodder produced with heat treatment. In the case of transformant cultivation on low-grade vegetable substrates, they also possessed confirmed phytase activity, as well as an increase in the content of intracellular phosphates as compared to cells of the wild-type Y. lipolytica strain.

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Funding

The study was performed within the framework of the State Assignments nos. 40.2713.2017/PCh (31.05.2017) and 0104-2019-0024.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071, Moscow, Russia

    E. G. Serdyuk, E. P. Isakova, N. N. Gessler, A. N. Antipov & Y. I. Deryabina

  2. Kursk State University, 305000, Kursk, Russia

    E. V. Trubnikova

Authors
  1. E. G. Serdyuk
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  2. E. P. Isakova
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  3. N. N. Gessler
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  4. E. V. Trubnikova
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  5. A. N. Antipov
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  6. Y. I. Deryabina
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Corresponding author

Correspondence to Y. I. Deryabina.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by N. Statsyuk

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Serdyuk, E.G., Isakova, E.P., Gessler, N.N. et al. Activity of Neutral Phytase from Obesumbacterium proteus in Recombinant Strains of Yarrowia lipolytica under Cultivation on Low-Grade Vegetable Substrate. Appl Biochem Microbiol 55, 549–555 (2019). https://doi.org/10.1134/S0003683819050120

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  • DOI: https://doi.org/10.1134/S0003683819050120

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