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A Strain Rhodococcus wratislaviensis КТ112-7 as a Basis for Bioregeneration of PCB-Contaminated Metal/Carbon Catalyst

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Abstract

A possibility of the bioregeneration of polychlorinated biphenyl-contaminated metal/carbon catalysts using aerobic bacteria as biological agents has been shown. Using the 5%-Pd/Sibunit catalyst contaminated by the products of the hydrodechlorinaton of the PCB commercial mixture of the Sovol brand and a strain of Rhodococcus wratislaviensis КТ112-7 it was proved that the application of the bacterial culture permits to rapidly clean the catalyst with the minimal technical and economic costs. It was established that the degree of the catalyst decontamination for 24 h with the help of the intact bacteria cells was equal to 99% (0.77 mg/mL). According to the gas-liquid chromatography, the R. wratislaviensis КТ112-7 strain decomposed all the components occurring on 5%-Pd/Sibunit after the reaction of PCB hydrodechlorination. The efficiency of the bacterial destruction made up 97% (0.76 mg/mL). The residual portion of contaminating compounds was adsorbed by the bacterial cells. Therefore, the R. wratislaviensis КТ112-7 strain can be used as a biological component in the systems for PCB-contaminated metal/carbon catalyst cleaning.

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ACKNOWLEDGMENTS

The authors are acknowledged to M.G. Pervova, Cand. Chem. Sci. (the Postovskii Institute for Organic Synthesis, Russ. Acad. Sci., Ural Branch, Ekaterinburg, Russia), for the assistance in the gas chromatography procedures. The work was performed within the framework of the state task on the theme “Search and Selection of New Promising Microorganisms for Biotechnology Purposes. Creation of Immunochemical Diagnostic Systems” (State Registration no. 01201353246).

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  1. Institute of Ecology and Genetics of Microorganisms, Perm’ Federal Scientific Center, Russian Academy of Sciences, Ural Branch, 614081, Perm’, Russia

    D. O. Egorova & V. A. Demakov

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  1. D. O. Egorova
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  2. V. A. Demakov
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Correspondence to D. O. Egorova.

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Abbreviations—AMU, atomic mass unit; GC-FID, gas chromatography with flame ionization detector; GC-MS, gas chromatography–mass spectrometry; IUPAC, International Union of Pure and Applied Chemistry; OD, optical density; P mixture, mixture of compounds occurring on catalyst particles and resulted from reaction of Sovol hydrodechlorination; PCB, polychlorinated biphenyls.

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Egorova, D.O., Demakov, V.A. A Strain Rhodococcus wratislaviensis КТ112-7 as a Basis for Bioregeneration of PCB-Contaminated Metal/Carbon Catalyst. Appl Biochem Microbiol 54, 876–885 (2018). https://doi.org/10.1134/S0003683818090119

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