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Gene Expression of the Antimicrobial Peptide Bombinin Increases the Resistance of Transgenic Tobacco Plants to Phytopathogens

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Abstract

Transgenic tobacco (Nicotiana tabacum L.) plants with an artificial gene from the antimicrobial peptide bombinin (bom) have been obtained and studied. The presence of the bom gene in the genome of kanamycin-resistant plants was shown by PCR. Expression of the bom gene was confirmed by antimicrobial activity measurements in leaf extracts. The obtained plants were morphogenetically resistant to Erwinia carotovora bacteria and Rhizoctonia solani fungi phytopathogens. In addition, the protective oxidative reaction to the infection, i.e., the SOD activity and proline content, were lower in transgenic plants than in the infected nontransgenic plants. Plants with the expression of the antimicrobial bombinin peptide gene are promising for use in agricultural biotechnology as plant protectors.

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ACKNOWLEDGMENTS

The work was financially supported by the Russian Fund for Basic Research, project nos. 15-08-02050 and 16-04-00623.

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute for Bioorganic Chemistry, Pushchino Branch, 142290, Pushchino, Russia

    N. S. Zakharchenko, S. V. Pigoleva, O. V. Furs, T. V. Shevchuk, O. V. Dyachenko & Ya. I. Buryanov

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  1. N. S. Zakharchenko
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  2. S. V. Pigoleva
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  3. O. V. Furs
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  4. T. V. Shevchuk
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  5. O. V. Dyachenko
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  6. Ya. I. Buryanov
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Correspondence to N. S. Zakharchenko.

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Translated by V. Mittova

Abbreviations: AMPs—antimicrobial peptides; ROS—reactive oxygen species; BSA—bovine serum albumin; PGA—potato glucose agar; MDA—malonic dialdehyde; PAAG—polyacrylamide gel; bp—base pair; POL—peroxide oxidation of lipids; PCR—polymerase chain reaction; SOD—superoxide dismutase; bom—bombinin gene; dNTP—deoxynucleoside triphosphate (s); 35S—35S promoter of cauliflower mosaic virus; SDS—sodium dodecyl sulfate.

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Zakharchenko, N.S., Pigoleva, S.V., Furs, O.V. et al. Gene Expression of the Antimicrobial Peptide Bombinin Increases the Resistance of Transgenic Tobacco Plants to Phytopathogens. Appl Biochem Microbiol 54, 730–735 (2018). https://doi.org/10.1134/S0003683818070086

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