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Carbohydrate Specificity of Antibodies Against Yeast Preparations of Saccharomyces cerevisiae and Candida krusei

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Abstract

Laboratory animals (the California rabbit) were immunized with natural preparations of Saccharomyces cerevisiae and Candida krusei yeasts. The resulting sera have been analyzed with ligand libraries constructed from synthetic oligosaccharides related to the main carbohydrate components of the fungal cell wall. It is shown that the antibodies in the studied sera predominantly recognize mannan fragments, with the carbohydrate specificity profile being different for S. cerevisiae and C. krusei. Sera against S. cerevisiae contained antibodies that recognize β-glucan, and the minimal recognizable epitope is linear trisaccharide. These results are necessary for the creation of needed immunoenzyme diagnostics for the detection and species characterization of fungi.

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REFERENCES

  1. Barnett, J.A., Payne, R.W., and Yarrow, D., Yeasts: Characteristics and Identification, Cambridge, United Kingdom: Cambridge University Press, 1990, 2 ed.

  2. Chernov, I.Yu., Drozhzhi v prirode (Yeasts in Nature), Moscow: Tovar. Nauch. Izd. KMK, 2013.

    Google Scholar 

  3. Glushakova, A.M. and Chernov, I.Y., Microbiology, 2010, vol. 79, no. 6, pp. 830–839.

    Article  CAS  Google Scholar 

  4. de Hoog, G.S., Guarro, J., Gené, J., and Figueras, M.J., Atlas of Clinical Fungi, Utrecht: Centraalbureau voor Schimmelcultures, ASM Press, 2000, 2nd ed.

  5. Latgé, J.P., Mol. Microbiol., 2007, vol. 66, no. 2, pp. 279–290.

    Article  CAS  PubMed  Google Scholar 

  6. Lipke, P.N. and Ovalle, R., J. Bacteriol., 1998, vol. 180, no. 15, pp. 3735–3740.

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Karelin, A.A., Tsvetkov, Y.E., and Nifantiev, N.E., Russ. Chem. Rev., 2017, vol. 86, no. 11, pp. 1073–1126.

    Article  CAS  Google Scholar 

  8. Krylov, V.B., Paulovičová, L., Paulovičová, E., Tsvetkov, Y.E., and Nifantiev, N.E., Pure Appl. Chem., 2017, vol. 89, no. 7, pp. 885–898.

    Article  CAS  Google Scholar 

  9. Oliveri, S., Trovato, L., Betta, P., Romeo, M.G., and Nicoletti, G., Clin. Microbiol. Infect., 2008, vol. 14, no. 4, pp. 391–393.

    Article  CAS  PubMed  Google Scholar 

  10. Fukazawa, Y., Shinoda, T., and Tsuchiya, T., J. Bacteriol., 1968, vol. 95, no. 3, pp. 754–763.

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Hall, R.A. and Gow, N.A., Mol. Microbiol., 2013, vol. 90, no. 6, pp. 1147–1161.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Rimek, D., Singh, J., and Kappe, R., J. Clin. Microbiol., 2003, vol. 41, no. 7, pp. 3395–3398.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Krylov, V.B., Argunov, D.A., Vinnitskiy, D.Z., Verkhnyatskaya, S.A., Gerbst, A.G., Ustyuzhanina, N.E., Dmitrenok, A.S., Huebner, J., Holst, O., Siebert, H.-C., and Nifantiev, N.E., Chem.-Eur. J., 2014, vol. 20, no. 50, pp. 16516–16522.

    Article  CAS  PubMed  Google Scholar 

  14. Komarova, B.S., Gerbst, A.G., Finogenova, A.M., Dmitrenok, A.S., Tsvetkov, Y.E., and Nifantiev, N.E., Org. Chem., 2017, vol. 82, no. 17, pp. 8897–8908.

    Article  CAS  Google Scholar 

  15. Varki, A., Cummings, R., Esko, J., Freeze, H., Hart, G., and Marth, J., Essentials of Glycobiology, New York: Cold Spring Harbor, 1999.

  16. Yashunsky, D.V., Tsvetkov, Y.E., Grachev, A.A., Chizhov, A.O., and Nifantiev, N.E., Carbohydr. Res., 2016, vol. 419, pp. 8–17.

    Article  CAS  PubMed  Google Scholar 

  17. Yashunsky, D.V., Tsvetkov, Y.E., and Nifantiev, N.E., Carbohydr. Res., 2016, vol. 436, pp. 25–30.

    Article  CAS  PubMed  Google Scholar 

  18. Krylov, V.B., Argunov, D.A., Solovev, A.S., Petruk, M.I., Gerbst, A.G., Dmitrenok, A.S., Shashkov, A.S., Latgé, J.-P., and Nifantiev, N.E., Org. Biomol. Chem., 2018, vol. 16, no. 7, pp. 1188–1199.

    Article  CAS  PubMed  Google Scholar 

  19. Gerbst, A.G., Grachev, A.A., Yashunsky, D.V., Tsvetkov, Y.E., Shashkov, A.S., and Nifantiev, N.E., J. Carbohydr. Chem., 2013, vol. 32, no. 3, pp. 205–221.

    Article  CAS  Google Scholar 

  20. Yudina, O.N., Tsvetkov, Yu.E., and Nifant’ev, N.E., Izv. Ross. Akad. Nauk, Ser. Khim., 2015, vol. 12, pp. 2932–2941.

    Google Scholar 

  21. Yudina, O.N., Tsvetkov, Yu.E., and Nifant’ev, N.E., Izv. Ross. Akad. Nauk, Ser. Khim., 2016, vol. 12, pp. 2937–2942.

    Google Scholar 

  22. Argunov, D.A., Krylov, V.B., and Nifantiev, N.E., Org. Biomol. Chem., 2015, vol. 13, no. 11, pp. 3255–3267.

    Article  CAS  PubMed  Google Scholar 

  23. Argunov, D.A., Krylov, V.B., and Nifantiev, N.E., Org. Lett., 2016, vol. 18, no. 21, pp. 5504–5507.

    Article  CAS  PubMed  Google Scholar 

  24. Komarova, B.S., Orekhova, M.V., Tsvetkov, Y.E., Beau, R., Aimanianda, V., Latge, J.P., and Nifantiev, N.E., Chem.-Eur. J., 2015, vol. 21, no. 3, pp. 1029–1035.

    Article  CAS  PubMed  Google Scholar 

  25. Krylov, V.B., Petruk, M.I., Grigor’ev, I.V., Lebedin, Yu.S., Glushko, N.I., Khaldeeva, E.V., Argunov, D.A., Khatuntseva, E.A., Toplishek, M.V., Komarova, B.S., Karelin, A.A., Yudina, O.N., Men’shov, V.M., Yashunskii, D.V., Tsvetkov, Yu.E., and Nifant’ev, N.E., Bioorg. Khim., 2018, vol. 44, no. 1, pp. 78–86.

    Google Scholar 

  26. Tsvetkov, Y.E., Burg-Roderfeld, M., Loers, G., Arda, A., Sukhova, E.V., Khatuntseva, E.A., Grachev, A.A., Chizhov, A.O., Siebert, H.-C., Schachner, M., Jimenez-Barbero, J., and Nifantiev, N.E., J. Am. Chem. Soc., 2012, vol. 134, no. 1, pp. 426–435.

    Article  CAS  PubMed  Google Scholar 

  27. Krylov, V.B., Petruk, M.I., Glushko, N.I., Khaldeeva, E.V., Mokeeva, V.L., Bilanenko, E.N., Lebedin, Yu.S., Eremin, S.A., and Nifant’ev, N.E., Appl. Biochem. Microbiol., 2018, vol. 45, no. 5, pp. 525–531.

    Google Scholar 

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Authors and Affiliations

  1. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    V. B. Krylov, M. I. Petruk, A. A. Karelin, D. V. Yashunuskii, Yu. E. Tsvetkov & N. E. Nifantiev

  2. Kazan Research Institute of Epidemiology and Microbiology, 420015, Kazan, Russia

    N. I. Glushko & E. V. Khaldeeva

  3. Lomonosov Moscow State University, 119991, Moscow, Russia

    V. L. Mokeeva, E. N. Bilanenko & S. A. Eremin

  4. XEMA Company Limited, 105264, Moscow, Russia

    Y. S. Lebedin

Authors
  1. V. B. Krylov
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  2. M. I. Petruk
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  3. A. A. Karelin
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  4. D. V. Yashunuskii
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  5. Yu. E. Tsvetkov
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  6. N. I. Glushko
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  7. E. V. Khaldeeva
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  8. V. L. Mokeeva
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  9. E. N. Bilanenko
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  10. Y. S. Lebedin
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  11. S. A. Eremin
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  12. N. E. Nifantiev
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Correspondence to N. E. Nifantiev.

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Translated by A. Ostyak

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Krylov, V.B., Petruk, M.I., Karelin, A.A. et al. Carbohydrate Specificity of Antibodies Against Yeast Preparations of Saccharomyces cerevisiae and Candida krusei. Appl Biochem Microbiol 54, 665–669 (2018). https://doi.org/10.1134/S0003683818060108

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  • DOI: https://doi.org/10.1134/S0003683818060108

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