Skip to main content
SpringerLink
Log in

Biosurfactant Production by Pseudomonas aeruginosa SNP0614 and its Effect on Biodegradation of Petroleum

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

An efficient biosurfactant-producing strain was isolated and cultured from Dagang oil field (China) using crude oil as sole source of carbon. Based on partial sequenced 16S rDNA analysis, the isolated strain was identified as Pseudomonas aeruginosa SNP0614. The bacterium P. aeruginosa SNP0614 produced a type of biosurfactant with excessive foam-forming properties. After microbial cultivation at 37°C and 150 rpm for 12 h, the produced biosurfactant was found to reduce the surface tension to 25.4 mN/m with critical micelle concentration (CMC) of 45.0 mg/L. After 20 days of incubation, the biosurfactant exhibited 90% emulsification activity (E24) on crude oil. FTIR spectroscopy of extracted biosurfactant indicated the biosurfactant as lipopeptide. The significant synergistic effect between P. aeruginosa SNP0614 and the mixed oildegrading bacteria resulted in increasing n-alkanes degradation rate by 30%. The strain P. aeruginosa SNP0614 represented as a promising biosurfactant producer and could be applied in a variety of biotechnological and industrial processes, particularly in microbial enhanced oil recovery and the bioremediation of oil pollution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sun, S., Luo, Y., Cao, S., Li, W., Zhang, Z., Jiang, L., et al., Bioresour. Technol., 2013, vol. 144, pp. 44–49.

    Article  CAS  PubMed  Google Scholar 

  2. Choi, H.J., Seo, J.Y., Hwang, S.M., Lee, Y.I., Jeong, Y.K., Moon, J.Y., and Joo, W.H., Biotechnol. Bioprocess Eng., 2013, vol. 18, no. 5, pp. 1000–1007.

    Article  CAS  Google Scholar 

  3. Ferradji, F. Z., Mnif, S., Badis, A., Rebbani, S., Fodil, D., Eddouaouda, K. and Sayadi, S., Int. Biodeterior. Biodegrad., 2014, vol. 86, pp. 300–308.

    Article  CAS  Google Scholar 

  4. Abbasi, H., Hamedi, M.M., Lotfabad, T.B., Zahiri, H. S., Sharafi, H., Masoomi, F., et al., J. Biosci. Bioeng., 2012, vol. 113, no. 2, pp. 211–219.

    Article  CAS  PubMed  Google Scholar 

  5. Wahaibi, A., Joshi, Y. S., Al–Bahry, S., Elshafie, A., Al–Bemani, A., and Shibulal, B., Colloids Surf. B, 2014, vol. 114, pp. 324–333.

    Article  Google Scholar 

  6. Lawniczak, L., Marecik, R., and Chrzanowski, L., Appl. Microbiol. Biotechnol., 2013, vol. 97, no. 6, pp. 2327–2339.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Jain, R.M., Mody, K., Joshi, N., Mishra, A., and Jha, B. Int. J. Biol. Macromol., 2013, vol. 62, no. 11, pp. 52–58.

    Article  CAS  PubMed  Google Scholar 

  8. Lotfabad, T. B., Shourian, M., Roostaazad, R., Najafabadi, A. R., Adelzadeh, M. R., and Noghabi, K. A. Colloids Surf. B, 2009, vol. 69, no. 2, pp.183–193.

    Article  CAS  Google Scholar 

  9. Chandankere, R., Yao, J., Choi, M.M., Masakorala, K., and Chan, Y. Biochem. Eng. J., 2013, vol. 74, pp. 46–53.

    Article  CAS  Google Scholar 

  10. Torres, L., Moctezuma, A., Avendaño, J.R., Muñoz, A., and Gracida, J., J. Pet. Sci. Eng., 2011, vol. 76, no. 1, pp. 6–11.

    Article  CAS  Google Scholar 

  11. Johannes, H., Claudia, M., Boris, K., Axel, K., Raphael, H., Frank Ki., et al., Appl. Microbiol. Biotechnol. 2014, vol. 98, no. 21, pp. 609–618.

    Google Scholar 

  12. Varjani S.J. and Upasani, V.N., Bioresour. Technol., 2017, vol. 232, pp. 389–397.

    Article  CAS  PubMed  Google Scholar 

  13. Kuttuvan, V.S., Rajeev, K.S., Lalitha, D.G., and Ashok P. Bioresour. Technol., 2015, vol. 191, pp. 133–139.

    Article  Google Scholar 

  14. de Oliveira, D.W, França, I.W., Félix, A.K., Martins, J.J., Giro, M.E., Melo, V.M., and Gonçalves, L.R. Colloids Surf. B, 2013, vol. 101, pp. 34–43.

    Article  Google Scholar 

  15. Mnif, I., Elleuch, M., Chaabouni, S.E., and Ghribi, D., Crop Prot., 2013, vol. 50, pp. 66–72.

    Article  CAS  Google Scholar 

  16. Aparna, A., Srinikethan, G., and Smitha, H., Colloids Surf. B, 2013, vol. 95, pp. 23–29.

    Article  Google Scholar 

  17. Hassanshahian, M., Emtiazi, G., and Cappello, S., Mar. Pollut. Bull., 2012, vol. 64, no. 1, pp. 7–12.

    Article  CAS  PubMed  Google Scholar 

  18. Rodrigues, L.R., Teixeira, J.A., VanderMei, H.C., and Oliveira, R., Colloids Surf. B, 2006, vol. 49, no. 1, pp. 79–86.

    Article  CAS  Google Scholar 

  19. Youssef, N.H., Duncan, K.E., Nagle, D.P., Savage, K.N., Knapp, R.M., and Mcinerney, M.J., J. Microbiol. Methods, 2004, vol. 56, no. 3, pp. 339–347.

    Article  CAS  PubMed  Google Scholar 

  20. Chandankere, R., Yao, J., Cai, M.M., Masakorala, K., Jain, A. and Choi M. M., Fuel, 2014, vol. 122, pp. 140–148.

    Article  CAS  Google Scholar 

  21. Cai, M.M., Yao, J., Yang, H., Wang, R., and Masakorala, K., Bioresour. Technol., 2013, vol. 144, pp. 100–106.

    Article  CAS  PubMed  Google Scholar 

  22. Zheng, S., Hu, J., Chen, K., Yao, J., Yu, Z., and Lin, X., Soil Biol. Biochem., 2009, vol. 41, no. 10, pp. 2094–2099.

    Article  CAS  Google Scholar 

  23. Aparna, A., Srinikethan, G., Smitha, H., Colloids Surf. B., 2012, vol. 95, pp. 23–29.

    Article  CAS  Google Scholar 

  24. Silva, S., Farias, C., Rufino, R., Luna, J., and Sarubbo, L., Colloids Surf. B., 2010, vol. 79, no. 2, pp. 174–183.

    Article  CAS  Google Scholar 

  25. Abbasi H., Hamedi M.M., Lotfabad T.B., Zahiri H.S., Sharafi H., Masoomi F., et al., J. Biosci. Bioeng., 2012, vol. 113, no. 2, pp. 211–219.

    Article  CAS  PubMed  Google Scholar 

  26. Nikolopoulou, M., N. Pasadakis, N., Norf, H., and Kalogerakis, N., Mar. Pollut. Bull., 2013, vol. 77, no. 1, pp. 37–44.

    Article  CAS  PubMed  Google Scholar 

  27. Li, Y.Q., Liu, H.F., Tian, Z.L., Zhu, L.H., Wu, Y.H. and Tang, H. Q. Biomed. Environ. Sci., 2008, vol. 21, no. 3, pp. 181–187.

    Article  CAS  PubMed  Google Scholar 

  28. Sriram, M.I., Gayathiri, S. Gnanaselvi, U., Jenifer, P.S., Mohan S., and Gurunathan, S., Bioresour. Technol., 2011, vol. 102, pp. 9291–9295.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Loess Plateau, Shan Xi University, Taiyuan, 030006, China

    W. J. Liu

  2. School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China

    X. D. Duan & L. P. Wu

  3. Department of Botany, Faculty of Science, University of Ruhuna, Matara, Sri Lanka

    K. Masakorala

Authors
  1. W. J. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. X. D. Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. P. Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Masakorala
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to W. J. Liu.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, W.J., Duan, X.D., Wu, L.P. et al. Biosurfactant Production by Pseudomonas aeruginosa SNP0614 and its Effect on Biodegradation of Petroleum. Appl Biochem Microbiol 54, 155–162 (2018). https://doi.org/10.1134/S0003683818020060

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683818020060

Keywords

Search

Navigation