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Study of the Conditions for the Biosynthesis of Bacterial Cellulose by the Producer Medusomyces gisevii Sa-12

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Abstract

The effect of culture conditions on the bacterial cellulose (BC) yield synthesized by symbiotic culture Medusomyces gisevii Sa-12 grown in synthetic nutrient medium was studied for the first time. The conditions providing the maximum yield of bacterial cellulose were evaluated: the initial glucose concentration in the culture medium was 20–25 g/L, the temperature was 24–27°C, the ratio of the nutrient medium to the air volume was 1: 10, and the content of the black tea extracts comprised 1.6–4.8 g/L. A sample of chemically pure BC with the following characteristics was obtained under the selected culture conditions: moisture— 99.0%, degree of polymerization—4800, average width of microfibrillar ribbons—30.6 nm, degree of crystallinity— 86%, and the content of triclinic modification Iα—98%.

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Authors and Affiliations

  1. Institute for Problems of Chemical and Energetic Technologies of the Siberian Branch of the Russian Academy of Sciences, Biysk, 659322, Russia

    E. K. Gladysheva, E. A. Skiba, V. N. Zolotukhin & G. V. Sakovich

Authors
  1. E. K. Gladysheva
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  2. E. A. Skiba
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  3. V. N. Zolotukhin
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  4. G. V. Sakovich
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Correspondence to E. K. Gladysheva.

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Original Russian Text © E.K. Gladysheva, E.A. Skiba, V.N. Zolotukhin, G.V. Sakovich, 2018, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2018, Vol. 54, No. 2, pp. 165–174.

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Gladysheva, E.K., Skiba, E.A., Zolotukhin, V.N. et al. Study of the Conditions for the Biosynthesis of Bacterial Cellulose by the Producer Medusomyces gisevii Sa-12. Appl Biochem Microbiol 54, 179–187 (2018). https://doi.org/10.1134/S0003683818020035

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  • DOI: https://doi.org/10.1134/S0003683818020035

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