Applied Biochemistry and Microbiology

, Volume 53, Issue 6, pp 661–668 | Cite as

Expression of the zebrafish β-defensin 3 mature peptide in Pichia pastoris and its purification and antibacterial activity

  • Y. TaoEmail author
  • C. F. Song
  • W. Li


Defensins are abundant in cells and tissues that are involved in host defense against microbial infections. zfDB3 (zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish (Danio rerio). Here we focus on mzfDB3, which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized mzfDB3 gene with a 6×His-tag at the 3′-end was inserted into the pPICZαA expression vector and transformed into Pichia pastoris X-33 cells. The recombinant zebrafish β-defensin 3 mature peptide (rmzfDB3) was induced with 1.0% methanol at 29°C for 72 h and purified by immobilized metal affinity chromatography. MALDI-TOF/TOF analysis confirmed the expected purified product (rmzfDB3, 5.9 kDa). Fermentation supernatant, which contained rmzfDB3, showed antibacterial activity against Grampositive (i.e., Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus) and Gram-negative (i.e., Escherchia coli BL21, Vibrio parahaemolyticus, Salmonella lignieres, and Pseudomonas aeruginosa) bacteria.


zebrafish β-defensin 3 mature peptide Pichia pastoris recombinant protein expression antibacterial activity 



zebrafish ß-defensin 3


zebrafish ß-defensin 3 mature peptide


recombinant zebrafish ß-defensin 3 mature peptide


immobilized metal affinity chromatography


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Copyright information

© Pleiades Publishing, Inc. 2017

Authors and Affiliations

  1. 1.College of Food Science and TechnologyShanghai Ocean UniversityShanghaiChina

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