Highly sensitive immunochromatographic test systems were developed for the detection of zearalenone (ZEA) and T-2 toxin (T2T) using magnetite nanoparticles (MNPs) for the labeling. In order to detect an analyte with high sensitivity, the competitive reaction was performed with free specific antibodies, while immune complexes were detected by the reaction with label-conjugated anti-species antibodies. The conditions for the synthesis of magnetite nanoparticles conjugated to anti-species antibodies were optimized. The concentrations of specific reagents that provided the highly sensitive detection of T-2 toxin and zearalenone were found. The instrumental detection limit for the determination of T-2 toxin and zearalenone in baby food samples (oat flakes) was 0.1 and 0.05 ng/mL (2.0 and 1.0 ng/g), respectively. The assay time was 15 min. The results of the present study confirm the possibility of the practical use of magnetite nanoparticles for immunochromatographic assay labeling.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Urusov, A.E., Zherdev, A.V., and Dzantiev, B.B., Appl. Biochem. Microbiol., 2010, vol. 46, no. 3, pp. 253–266.
Dzantiev, B.B., Byzova, N.A., Urusov, A.E., and Zherdev, A.V., Trend. Anal. Chem., 2014, vol. 55, pp. 81–93.
O’Farrell, B., in Lateral Flow Immunoassay Systems: Evolution from the Current State of the Art to the Next Generation of Highly Sensitive, Quantitative Rapid Assays, Wild, D., Ed., Oxford Elsevier, 2013.
Ching, K.H., in Lateral Flow Immunoassay, Hnasko, R., Ed., New York Springer, 2015.
Anfossi, L., Baggiani, C., Giovannoli, C., D’Arco, G., and Giraudi, G., Anal. Bioanal. Chem., 2013, vol. 405, nos. 2–3, pp. 467–480.
Köppen, R., Koch, M., Siegel, D., Merkel, S., Maul, R., and Nehls, I., Appl. Microbiol. Biotechnol., 2010, vol. 86, no. 6, pp. 1595–1612.
Urusov, A.E., Zherdev, A.V., and Dzantiev, B.B., Microkhim. Acta, 2014, vol. 181, nos. 15–16, pp. 1939–1946.
Urusov, A.E., Petrakova, A.V., Zherdev, A.V., and Dzantiev, B.B., Biosens. Bioelectron, 2016, vol. 86, pp. 575–579.
Urusov, A.E., Kostenko, S.N., Sveshnikov, P.G., Zherdev, A.V., and Dzantiev, B.B., Sensor. Actuat., 2011, vol. 156, no. 1, pp. 343–349.
Kong, D., Liu, L., Song, S., Suryoprabowo, S., Li, A., Kuang, H., Wang, L., and Xu, C., Nanoscale, 2016, vol. 8, no. 9, pp. 5245–5253.
Orlov, A.V., Bragina, V.A., Nikitin, M.P., and Nikitin, P.I., Biosens. Bioelectron., 2016, vol. 79, pp. 423–429.
Zheng, C., Wang, X., Lu, Y., and Liu, Y., Food Control, 2012, vol. 26, no. 2, pp. 446–452.
Petrakova, A.V., Urusov, A.E., Zherdev, A.V., and Dzantiev, B.B., Vestnik Mosk. Univ. Ser. 2: Khim., 2015, vol. 56, no. 6, pp. 372–378.
Song, S., Liu, N., Zhao, Z., Njumbe, EdiageE., Wu, S., Sun, C., De Saeger, S., and Wu, A., Anal. Chem., 2014, vol. 86, no. 10, pp. 4995–5001.
Zhang, X., Wang, X., Sun, M., Zhang, X., Song, H., Yan, Y., Sun, J., Li, X., and Fang, W., Toxins, 2015, vol. 7, no. 10, pp. 4216–4231.
Original Russian Text © A.V. Petrakova, A.E. Urusov, A.V. Zherdev, L. Liu, C. Xu, B.B. Dzantiev, 2017, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 4, pp. 420–426.
About this article
Cite this article
Petrakova, A.V., Urusov, A.E., Zherdev, A.V. et al. Application of magnetite nanoparticles for the development of highly sensitive immunochromatographic test systems for mycotoxin detection. Appl Biochem Microbiol 53, 470–475 (2017). https://doi.org/10.1134/S0003683817040111
- magnetite nanoparticles
- immunochromatographic assay
- sensitivity enhancement
- test strips
- T-2 toxin