Abstract
A method has been developed for the quantitative estimation of the binding force of a model microsphere with a eukaryocyte based on the optical trap in order to study the molecular mechanism of adhesion between an individual bacterium and a host cell. The substantial role of LPS O-side chains in the adhesiveness of Yersinia pseudotuberculosis 1b to J774 macrophages has been revealed with the use of a set of microspheres functionalized with lipopolysaccharide (LPS) preparations and antibodies with different specificities. The results indicate the significance of the O-antigen as a pathogenicity factor of Y. pseudotuberculosis in colonization of a macroorganism. The developed methodical approaches can be applied to the study of molecular mechanisms of the pathogenesis of pseudotuberculosis and other infectious diseases to improve antiepidemic service.
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Kline, K.A., Falker, S., Dahlberg, S., Normark, S., and Henriques-Normark, B., Cell Host Microbe, 2009, vol. 5(6), pp. 580–592.
Zav’yalov, V., Zavialov, A., Zav’yalova, G., and Korpela, T., FEMS Microbiol. Rev., 2010, vol. 34(3), pp. 317–378.
Mikula, K.M., Kolodziejczyk, R., and Goldman, A., Front. Cell. Infect. Microbiol., 2012, vol. 2, pp. 1–14.
Pizarro-Cerdá, J. and Cossart, P., Cell, 2006, vol. 124(4), pp. 715–727.
Razatos, A., Ong, Y.L., Sharma, M.M., and Georgiou, G., Proc. Natl. Acad. Sci. U. S. A., 1998, vol. 95(19), pp. 11059–11064.
Boyle, E.C. and Finlay, B.B., Curr. Opin. Cell. Biol., 2003, vol. 15(5), pp. 633–639.
Krachler, A.M. and Orth, K., Microbe, 2013, vol. 8, pp. 286–290.
Miller, V.L., Beer, K.B., Heusipp, G., Young, B.M., and Wachtel, M.R., Mol. Microbiol., 2001, vol. 41(5), pp. 1053–1062.
Yang, Y., Merriam, J.J., Mueller, J.P., and Isberg, R.R., Infect. Immun., 1996, vol. 64(7), pp. 2483–2489.
Felek, S., Lawrenz, M.B., and Krukonis, E.S., Microbiology, 2008, vol. 154(6), pp. 1802–1812.
Lawrenz, M.B., Lenz, J.D., and Miller, V.L., Infect. Immun., 2009, vol. 77(1), pp. 317–326.
Nair, M.K.M., De Masi, L., Yue, M., Galván, E.M., Chen, H., Wang, F., and Schifferli, D.M., Infect. Immun., 2015, vol. 83(5), pp. 1809–1819.
Eitel, J. and Dersch, P., Infect. Immun., 2002, vol. 70(9), pp. 4880–4891.
Isberg, R.R., Voorhis, D.L., and Falkow, S., Cell, 1987, vol. 50(5), pp. 769–778.
Felek, S., Tsang, T.M., and Krukonis, E.S., Infect. Immun., 2010, vol. 78(8), pp. 4134–4150.
Lahteenmaki, K., Kukkonen, M., and Korhonen, T.K., FEBS Lett., 2001, vol. 504(1-2), pp. 69–72.
Goguen, J.D., Bugge, T., and Degen, J.L., Methods, 2001, vol. 21(2), pp. 179–183.
Leo, J.C. and Skurnik, M., Adv. Exp. Med. Biol., 2011, vol. 715, pp. 1–15.
Lu, Q., Wang, J., Faghihnejad, A., Zeng, H., and Liu, Y., Soft Matter, 2011, vol. 7, pp. 9366–9379.
Amro, N.A., Kotra, L.P., Wadu-Mesthrige, K., Bulychev, A., Mobashery, S., and Liu, G., Langmuir, 2000, vol. 16(6), pp. 2789–2796.
Kukkonen, M., Suomalainen, M., Kyllönen, P., Lähteenmäki, K., Lång, H., Virkola, R., Helander, I.M., and Korhonen, T.K., Mol. Microbiol., 2004, vol. 51(1), pp. 215–225.
Skurnik, M. and Bengoechea, J.A., Carbohydr. Res., 2003, vol. 338(23), pp. 2521–2529.
Tsang, T.M., Wiese, J.S., Felek, S., Kronshage, M., and Krukonis, E.S., PLOS One, 2013, vol. 8(12), e83621.
Strauss, J., Burnham, N.A., and Camesano, T.A., J. Mol. Recogn., 2009, vol. 22(5), pp. 347–355.
Ivanov, I.E., Kintz, E.N., Porter, L.A., Goldberg, J.B., Burnham, N.A., and Camesano, T.A., J. Bacteriol., 2011, vol. 193(5), pp. 1259–1266.
Jones, J.F., Feick, J.D., Imoudu, D., Chukwumah, N., Vigeant, M., and Velegol, D., Appl. Environ. Microbiol., 2003, vol. 69(11), pp. 6515–6519.
Burks, G.A., Velegol, S.B., Paramonova, E., Lindenmuth, B.E., Feick, J.D., and Logan, B.E., Langmuir, 2003, vol. 19(6), pp. 2366–2371.
Targosz, M., Czuba, P., Biedron, R., Strus, M., Gamian, A., Marcinkiewicz, J., and Szymonski, M., Acta Phys. Pol. A, 2006, vol. 109(3), pp. 421–426.
Targosz, M., Labuda, A., Czuba, P., Biedron, R., Strus, M., Gamian, A., Marcinkiewicz, J., and Szymonski, M., Nanomedicine, 2006, vol. 2(2), pp. 82–88.
Wei, M.T., Hua, K.F., Hsu, J., Karmenyan, A., Tseng, K.Y., Wong, C.H., Hsu, H.Y., and Chiou, A., Opt. Express, 2007, vol. 15(17), pp. 11020–11032.
Westphal, O. and Jann, K., Methods Carbohydr. Chem., 1965, vol. 5, pp. 83–91.
Byvalov, A.A., Dudina, L.G., Litvinets, S.G., Novikova, O.D., Khomenko, O.D., Portnyagina, O.Yu., and Ovodov, Yu.S., Appl. Biochem. Microbiol., 2014, vol. 50(2), pp. 179–186.
Byvalov, A.A., Dudina, L.G., Chernyad’ev, A.V., Konyshev, I.V., Litvinets, S.G., and Ovodov, Yu.S., Mol. Gen. Mikrobiol. Virusol., 2015, vol. 30(2), pp. 93–100.
Wozniak, A., Mameren, J.V., and Ragona, S., Curr. Pharm. Biotechnol., 2009, vol. 10(5), pp. 467–473.
Silverman, B.W., Density Estimation for Statistics and Data Analysis, London Chapman and Hall, 1986.
Huber, M., Kalis, C., Keck, S., Jiang, Z., Georgel, P., Du, X., Shamel, L., Sovath, S., Mudd, S., and Beutler, B., Eur. J. Immunol., 2006, vol. 36(3), pp. 701–711.
Sidorin, E.V., Kim, N.Yu., Leichenko, E.V., Anastyuk, S.D., Dmitrenok, P.S., Naberezhnykh, G.A., and Solov’eva, T.F., Biochemistry (Moscow), 2006, vol. 71(11), pp. 1278–1283.
Makoveichuk, E., Cherepanov, P., Lundberg, S., Forsberg, A., and Olivecrona, G.J., Lipid Res., 2003, vol. 44(2), pp. 320–330.
Skurnik, M., Adv. Exp. Med. Biol., 2003, vol. 529, pp. 187–197.
Knirel, Y.A. and Anisimov, A.P., Acta Naturae, 2012, vol. 4(3), pp. 46–58.
Holst, O., Adv. Exp. Med. Biol., 2003, vol. 529, pp. 219–228.
Somov, G.P., Pokrovskii, V.I., Besednova, N.N., and Antonenko, F.F., Psevdotuberkulez (Pseudotuberculosis), Moscow Meditsina, 2001.
Bengoechea, J.A., Adv. Exp. Med. Biol., 2003, vol. 529, pp. 267–274.
Bengoechea, J.A., Najdenski, H., and Skurnik, M., Mol. Microbiol., 2004, vol. 52(2), pp. 451–469.
Tang, K., Guo, H., Yi, W., Tsai, M., and Wang, P.G., Biochemistry, 2007, vol. 46(42), pp. 11744–11752.
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Original Russian Text © A.A. Byvalov, V.L. Kononenko, I.V. Konyshev, 2017, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 2, pp. 234–243.
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Byvalov, A.A., Kononenko, V.L. & Konyshev, I.V. Effect of lipopolysaccharide O-side chains on the adhesiveness of Yersinia pseudotuberculosis to J774 macrophages as revealed by optical tweezers. Appl Biochem Microbiol 53, 258–266 (2017). https://doi.org/10.1134/S0003683817020077
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DOI: https://doi.org/10.1134/S0003683817020077