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Synthesis of high-molecular-mass polyhydroxybutyrate from methanol in Methyloligella halotolerans C2

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Abstract

The influence of the concentrations of carbon and energy sources (methanol) and nitrogen (NH +4 ) on the yield and molecular mass (Mm) of poly-β-hydroxybutyrate (PHB) synthesized by the novel methylotroph Methyloligella halotolerans C2 was investigated. It was shown that the maximum concentrations of NH +4 and methanol in the medium for PHB biosynthesis were 0.15 g/L and 1 ± 0.2 mL/L, respectively. A unique high-molecular (8000–10000 kDa) PHB was obtained with NH +4 and methanol concentrations of 0.12 g/L and 1.25 mL/L, respectively, in a fermenter. The main physicochemical and mechanical properties of the obtained bioplastic with a molecular mass of 10000 kDa were determined.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    V. A. Ezhov, N. V. Doronina, M. N. Shmareva & Yu. A. Trotsenko

Authors
  1. V. A. Ezhov
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  2. N. V. Doronina
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  3. M. N. Shmareva
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  4. Yu. A. Trotsenko
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Correspondence to Yu. A. Trotsenko.

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Original Russian Text © V.A. Ezhov, N.V. Doronina, M.N. Shmareva, Yu.A. Trotsenko, 2017, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 1, pp. 55–60.

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Ezhov, V.A., Doronina, N.V., Shmareva, M.N. et al. Synthesis of high-molecular-mass polyhydroxybutyrate from methanol in Methyloligella halotolerans C2. Appl Biochem Microbiol 53, 47–51 (2017). https://doi.org/10.1134/S0003683817010112

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  • DOI: https://doi.org/10.1134/S0003683817010112

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