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Covalent immobilization of D-aminoacylase of strain Rhodococcus armeniensis AM6.1 and the characteristics of the biocatalyst

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Abstract

Immobilization of D-aminoacylase of the strain Rhodococcus armeniensis AM6.1 was carried out on the silochrome C-80 with a yield of enzymatic activity of 20%. The temperature and pH optima, thermal stability and dependence of thermal stability on pH for free and immobilized enzymes were compared. The possibility of using free and immobilized D-aminoacylases to produce D-amino acids from their racemic mixtures was demonstrated.

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Authors and Affiliations

  1. SPC “Armbiotechnology” NAS RA, Yerevan, 0056, Armenia

    A. A. Hambardzumyan, A. V. Mkhitaryan, A. M. Paloyan & S. A. Dadayan

Authors
  1. A. A. Hambardzumyan
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  2. A. V. Mkhitaryan
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  3. A. M. Paloyan
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  4. S. A. Dadayan
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Corresponding author

Correspondence to A. A. Hambardzumyan.

Additional information

Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 1, pp. 26–30.

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Hambardzumyan, A.A., Mkhitaryan, A.V., Paloyan, A.M. et al. Covalent immobilization of D-aminoacylase of strain Rhodococcus armeniensis AM6.1 and the characteristics of the biocatalyst. Appl Biochem Microbiol 53, 20–24 (2017). https://doi.org/10.1134/S0003683817010057

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  • DOI: https://doi.org/10.1134/S0003683817010057

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