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Development of thermotolerant bacterial consortium, the basis for biopreparation for remediation of petroleum-contaminated soils and waters in hot climates

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Abstract

The consortium of thermotolerant petroleum-oxidizing bacteria containing strains Gordonia sp. 1D VKM Ac-2720 D, Rhodococcus sp. Par7 VKM Ac-2722 D, and R. pyridinivorans L5A-BSU VKM Ac-2721 for destruction of oil and oil products in hot climates was developed for the first time. The consortium was effective in soils and liquid media at temperature as high as 50°C, at salinity up to 7%, and soil moisture of about 10%. The efficiency of petroleum destruction for 21 days was 70 and 59% at 24 and 45°C, respectively. The consortium of thermotolerant petroleum-destructing strains could be used as basis for the biopreparation for remediation of petroleum-contaminated soils and waters in hot climates.

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Abbreviations

DF:

diesel fuel

IR:

infrared

CFU:

colonyforming units

PAHs:

polycyclic aromatic hydrocarbons

bp:

base pairs

PCR:

polymerase chain reaction

LB medium:

Luria–Bertani nutrient medium

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Authors and Affiliations

  1. Pushchino State Natural Science Institute, Pushchino, 142290, Russia

    Ya. A. Delegan & A. E. Filonov

  2. Skryabin Institute for Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), Pushchino, 142290, Russia

    Ya. A. Delegan, A. A. Vetrova & A. E. Filonov

  3. Belorussian State University, Minsk, 220030, Belarus

    M. A. Titok

Authors
  1. Ya. A. Delegan
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  2. A. A. Vetrova
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  3. M. A. Titok
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  4. A. E. Filonov
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Correspondence to A. E. Filonov.

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Original Russian Text © Ya.A. Delegan, A.A. Vetrova, M.A. Titok, A.E. Filonov, 2016, published in Biotekhnologiya, 2016, No. 1, pp. 53–64.

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Delegan, Y.A., Vetrova, A.A., Titok, M.A. et al. Development of thermotolerant bacterial consortium, the basis for biopreparation for remediation of petroleum-contaminated soils and waters in hot climates. Appl Biochem Microbiol 52, 828–836 (2016). https://doi.org/10.1134/S0003683816090039

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