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Molecular-genetic and biochemical characteristics of citrate synthase from the citric-acid producing fungus Aspergillus niger

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Abstract

This review is devoted to achievements in the study of citrate synthase, an enzyme that plays a key role in cell metabolism and catalyzes the process of citric acid synthesis at the first step of the tricarboxylic acid cycle (TCA). The primary industrial producer of citric acid is Aspergillus niger fungus; therefore, the fundamental molecular-genetic and biochemical characteristics of the enzyme from this producer are discussed in comparison with those of other eukaryotes. The information on the mechanisms of the citric acid overproduction in A. niger and its evolutionary features is analyzed. The current review can be helpful in the development of a strategy for the design of more effective producers of organic acids.

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Abbreviations

MEC:

multi-enzyme complex

PAGE:

poly-acrylamide gel electrophoresis

bp:

base pairs

CS:

citrate synthase

TCA:

tricarboxylic acid cycle

AMP, ADP, ATP:

adenosine monophosphate, diphosphate, triphosphate, respectively

NAD, NADP, NAD+, NADH, NADPH:

nicotinamide adenine dinucleotide and its phosphorylated form, reduced form, oxidized form, phosphorylated reduced form, respectively

ORF:

open reading frame

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. St. Petersburg State Chemical-Pharmaceutical Academy, St. Petersburg, 197376, Russia

    K. V. Alekseev & V. P. Komov

  2. The St. Petersburg Academic University, St. Petersburg, 194021, Russia

    M. V. Dubina

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  1. K. V. Alekseev
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Original Russian Text © K.V. Alekseev, M.V. Dubina, V.P. Komov, 2016, published in Biotekhnologiya, 2016, No. 1, pp. 11–20.

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Alekseev, K.V., Dubina, M.V. & Komov, V.P. Molecular-genetic and biochemical characteristics of citrate synthase from the citric-acid producing fungus Aspergillus niger . Appl Biochem Microbiol 52, 810–817 (2016). https://doi.org/10.1134/S0003683816090027

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