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Comparison of L-lactate dehydrogenases of different origins produced in the cells of yeast Schizosaccharomyces pombe

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Abstract

The expression of L-lactate dehydrogenase genes ldh1 (Bos taurus), ldhA (Homo sapiens), ldhA (Rhizopus oryzae), ldh1 (Lactobacillus plantarum), and ldh1 (Lactobacillus pentosus) in the cells of yeast Schizosaccharomyces pombe VKPM U-3106 has been investigated. The catalytic characteristics of the enzymes encoded by these genes have been compared, and the intensity of lactic acid synthesis by the recombinant strains obtained has been evaluated. The enzymatic activity of L-lactate dehydrogenases from L. plantarum and L. pentosus was the highest (approximately 2 to 2.5 times higher than that of the mammalian enzymes), and these enzymes therefore appear to have the highest potential for the development of lactic-acid producing strains of yeast S. pombe.

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Abbreviations

HPLC:

high performance liquid chromatography

DTT:

dithiothreitol

CL:

culture liquid

LA:

lactic acid

OD:

optical density

bp:

base pairs

PCR:

polymerase chain reaction

LDH:

lactate dehydrogenase

ldh :

LDH gene

NADH:

reduced nicotinamide adenine dinucleotide

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Authors and Affiliations

  1. State Research Institute of Genetics and Selection of Industrial Microorganisms (GosNIIgenetika), Moscow, 117545, Russia

    L. N. Borshchevskaya, T. L. Gordeeva & S. P. Sineoky

Authors
  1. L. N. Borshchevskaya
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  2. T. L. Gordeeva
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  3. S. P. Sineoky
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Corresponding author

Correspondence to L. N. Borshchevskaya.

Additional information

Original Russian Text © L.N. Borshchevskaya, T.L. Gordeeva, S.P. Sineoky, 2015, published in Biotekhnologiya, 2015, No. 6, pp. 25–34.

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Borshchevskaya, L.N., Gordeeva, T.L. & Sineoky, S.P. Comparison of L-lactate dehydrogenases of different origins produced in the cells of yeast Schizosaccharomyces pombe . Appl Biochem Microbiol 52, 760–768 (2016). https://doi.org/10.1134/S0003683816080020

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  • DOI: https://doi.org/10.1134/S0003683816080020

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