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Fluorescence polarization immunoassay of ractopamine

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Abstract

A technique was developed for fluorescence polarization immunoassay (FPIA) of ractopamine, a toxic low molecular weight nonsteroidal growth regulator belonging to the most controlled contaminants of food products of animal origin. The assay is based on the competition between a sample containing ractopamine and ractopamine–fluorophore conjugate for binding to antibodies. The competition is monitored via changes in the degree of fluorescence polarization for plane-polarized excitation light, which differs for the free and antibody-bound forms of the conjugate. The optimal assay conditions were established, ensuring a high accuracy and minimal detection limit. The developed assay demonstrated a detection limit of 1 ng/mL and a range of detectable concentrations of 2.3–50 ng/mL, which met the requirements of sanitary control. The duration of the analysis was 10 min. The possible application of the developed FPIA was demonstrated with testing of turkey meat. The speed and simplicity of the proposed assay define its efficiency as a screening tool for safety of foods.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russia

    E. A. Zvereva, N. A. Shpakova, A. V. Zherdev & B. B. Dzantiev

  2. School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China

    L. Liu & C. Xu

  3. Department of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia

    S. A. Eremin

Authors
  1. E. A. Zvereva
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  2. N. A. Shpakova
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  3. A. V. Zherdev
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  4. L. Liu
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  5. C. Xu
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  6. S. A. Eremin
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  7. B. B. Dzantiev
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Corresponding author

Correspondence to B. B. Dzantiev.

Additional information

Original Russian Text © E.A. Zvereva, N.A. Shpakova, A.V. Zherdev, L. Liu, C. Xu, S.A. Eremin, B.B. Dzantiev, 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, Vol. 52, No. 6, pp. 632–638.

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Zvereva, E.A., Shpakova, N.A., Zherdev, A.V. et al. Fluorescence polarization immunoassay of ractopamine. Appl Biochem Microbiol 52, 673–678 (2016). https://doi.org/10.1134/S0003683816060168

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  • DOI: https://doi.org/10.1134/S0003683816060168

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