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Inducing cell wall-bound phenolic compounds by elicitors in eggplant (Solanum melongena)

Abstract

We investigated the effect of elicitation on cell wall strengthening in eggplant roots caused by 6 elicitors viz., chitosan (CH), salicylic acid (SA), methyl jasmonate, methyl salicylate and vitamins B2 and B12. Analysis of phenolic metabolites from eggplant roots by HPLC revealed presence of 6 major cell wall-bound phenolic compounds. They were 4-hydroxybenzoic acid (4-HBA), vanillic acid (VA), 4-hydroxybenzaldehyde (4-HBAld), vanillin (VAN), 4-coumaric acid (4-CA) and ferulic acid (FA). In eggplant roots, the concentrations of FA, VA and 4-HBA were 188.71, 113.64 and 109.42 μg/g DW, respectively, and they were higher than those of 4-HBAld, VAN and 4-CA. When elicited roots were analyzed by HPLC, quantitative differences could be clearly discerned in the amount of the phenolic compounds. After 48 h post-elicitation (hpe) in the presence of CH, the increase in 4-HBA, 4-CA and FA contents in cell wall was 2.6-, 2.8- and 3.0-fold, respectively, compared with control. After 72 hpe, in the presence of SA, the increase in 4-HBA, 4-CA and FA levels was 3.5-, 2.9- and 3.8-fold, respectively, compared with the control. As the elicitors have specific receptors in plants, it may be possible to utilize CH and SA for inducing resistance against important diseases in eggplant.

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Correspondence to S. Mandal.

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Mandal, S., Gupta, C.K. Inducing cell wall-bound phenolic compounds by elicitors in eggplant (Solanum melongena). Appl Biochem Microbiol 52, 650–656 (2016). https://doi.org/10.1134/S0003683816060120

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  • DOI: https://doi.org/10.1134/S0003683816060120

Keywords

  • chitosan
  • eggplant
  • ferulic acid
  • 4-hydroxybenzoic acid
  • salicylic acid
  • vitamin B2