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Inducing cell wall-bound phenolic compounds by elicitors in eggplant (Solanum melongena)

Applied Biochemistry and Microbiology volume 52, pages 650–656 (2016)Cite this article

Abstract

We investigated the effect of elicitation on cell wall strengthening in eggplant roots caused by 6 elicitors viz., chitosan (CH), salicylic acid (SA), methyl jasmonate, methyl salicylate and vitamins B2 and B12. Analysis of phenolic metabolites from eggplant roots by HPLC revealed presence of 6 major cell wall-bound phenolic compounds. They were 4-hydroxybenzoic acid (4-HBA), vanillic acid (VA), 4-hydroxybenzaldehyde (4-HBAld), vanillin (VAN), 4-coumaric acid (4-CA) and ferulic acid (FA). In eggplant roots, the concentrations of FA, VA and 4-HBA were 188.71, 113.64 and 109.42 μg/g DW, respectively, and they were higher than those of 4-HBAld, VAN and 4-CA. When elicited roots were analyzed by HPLC, quantitative differences could be clearly discerned in the amount of the phenolic compounds. After 48 h post-elicitation (hpe) in the presence of CH, the increase in 4-HBA, 4-CA and FA contents in cell wall was 2.6-, 2.8- and 3.0-fold, respectively, compared with control. After 72 hpe, in the presence of SA, the increase in 4-HBA, 4-CA and FA levels was 3.5-, 2.9- and 3.8-fold, respectively, compared with the control. As the elicitors have specific receptors in plants, it may be possible to utilize CH and SA for inducing resistance against important diseases in eggplant.

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Authors and Affiliations

  1. Plant Pathology Laboratory, Central Horticultural Experiment Station (ICAR-IIHR), Aiginia, Bhubaneswar, 751019, India

    S. Mandal & C. K. Gupta

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  1. S. Mandal
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  2. C. K. Gupta
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Correspondence to S. Mandal.

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Mandal, S., Gupta, C.K. Inducing cell wall-bound phenolic compounds by elicitors in eggplant (Solanum melongena). Appl Biochem Microbiol 52, 650–656 (2016). https://doi.org/10.1134/S0003683816060120

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  • DOI: https://doi.org/10.1134/S0003683816060120

Keywords

  • chitosan
  • eggplant
  • ferulic acid
  • 4-hydroxybenzoic acid
  • salicylic acid
  • vitamin B2