Skip to main content
SpringerLink
Log in

Resistance to cellobiose lipids and specific features of lipid composition in yeast

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

The significance of the fatty acid composition and ergosterol content in cells for resistance to cellobiose lipids has been investigated in the cells of mutant Saccharomyces cerevisiae strains that are unable to produce ergosterol or sphingomyelin and in the cells of microorganisms that produce cellobiose lipids. S. cerevisiae mutants were shown to be less sensitive to cellobiose lipids from Cryptococcus humicola than the wild-type strain, and the strains that produced cellobiose lipids were virtually insensitive to this compound as well. The sensitivity of Pseudozyma fusiformata yeast to its own cellobiose lipids was reduced under conditions that favored the production of these compounds. No correlation between the content of ergosterol and sensitivity to cellobiose lipids was observed in S. cerevisiae or in the strains that produced cellobiose lipids. The ratio between the levels of saturated and unsaturated fatty acids in the cells of the mutant strains was correlated to the sensitivity of the cells to cellobiose lipids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Golubev, W.I. and Shabalin, Y., FEMS Microbiol. Letts., 1994, vol. 119, no. 1, pp. 105–110.

    Article  CAS  Google Scholar 

  2. Puchkov, E.O., Zahringer, U., Lindner, B., Kulakovskaya, T.V., Seydel, U., and Wiese, A., Biochim. Biophys. Acta (Biomembranes), 2002, vol. 1558, no. 2, pp. 161–170.

    Article  CAS  Google Scholar 

  3. Kulakovskaya, T.V., Shashkov, A.S., Kulakovskaya, E.V., and Golubev, W.I., FEMS Yeast Res., 2005, vol. 5, no. 10, pp. 919–923.

    Article  CAS  PubMed  Google Scholar 

  4. Kulakovskaya, T.V., Kulakovskaya, E.V., and Golubev, W.I., FEMS Yeast Res., 2003, vol. 3, no. 4, pp. 401–404.

    Article  CAS  PubMed  Google Scholar 

  5. Kulakovskaya, E.V., Ivanov, A.Yu., Kulakovskaya, T.V., Vagabov, V.M., and Kulaev, I.S., Microbiology (Moscow), 2008, vol. 77, no. 3, pp. 288–292.

    Article  CAS  Google Scholar 

  6. Kulakovskaya, E.V., Vagabov, V.M., Ivanov, A.Yu., Trilisenko, L.V., Kulakovskaya, T.V., and Kulaev, I.S., Microbiology (Moscow), 2011, vol. 80, no. 1, pp. 10–14.

    Article  CAS  Google Scholar 

  7. Kulakovskaya, T.V., Shashkov, A.S., Kulakovskaya, E.V., Golubev, W.I., Zinin, A.I., Tsvetkov, Y.E., Grachev, A.A., and Nifantiev, N.E., J.Oleo Sci., 2009, vol. 58, no. 3, pp. 133–140.

    Article  CAS  PubMed  Google Scholar 

  8. Brajtburg, J., Powderly, W.G., Kobayashi, G.S., and Medoff, G., Antimicrob. Agents Chemother., 1990, vol. 34, no. 2, pp. 183–188.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Gray, K.C., Palacios, D.S., Dailey, I., Endo, M.M., Uno, B.E., Wilcock, B.C., and Burke, M.D., Proc. Natl. Acad. Sci. U. S. A., 2012, vol. 109, no. 7, pp. 2234–2239.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Borjihan, H., Ogita, A., Fujita, K., Hirasawa, E., and Tanaka, T., J. Antibiot. (Tokyo), 2009, vol. 62, no. 12, pp. 691–697.

    Article  CAS  Google Scholar 

  11. Ren, B., Dai, H.-Q., Pei, G., Tong, Y.-J., Zhuo, Y., Yang, N., Su, M.-Y., Huang, P., Yang, Y.-Z., and Zhang, L.-X., Appl. Microbiol. Biotechnol., 2014, vol. 98, no. 6, pp. 2609–2616.

    Article  CAS  PubMed  Google Scholar 

  12. Silva, L., Coutinho, A., Fedorov, A., and Prieto, M., Biochim. Biophys. Acta, 2006, vol. 1758, no. 4, pp. 452–459.

    Article  CAS  PubMed  Google Scholar 

  13. Semis, R., Kagan, S., Berdicevsky, I., Polacheck, I., and Segal, E., Med. Mycol., 2013, vol. 51, no. 4, pp. 422–431.

    Article  CAS  PubMed  Google Scholar 

  14. Kristanc, L., Bozic, B., and Gomiscek, G., Biochim. Biophys. Acta, 2014, vol. 1838, no. 10, pp. 2635–2645.

    Article  CAS  PubMed  Google Scholar 

  15. Mimee, B., Pelletier, R., and Belanger, R.R., J. Appl. Microbiol., 2009, vol. 107, no. 3, pp. 989–996.

    Article  CAS  PubMed  Google Scholar 

  16. Younsi, M., Ramanandraibe, E., Bonaly, R., Donner, M., and Coulon, J., Antimicrob. Agents Chemother., 2000, vol. 44, no. 7, pp. 1911–1916.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Milgrom, E., Diab, H., Middleton, F., and Kane, P.M., J. Biol. Chem., 2007, vol. 282, no. 10, pp. 7125–7136.

    Article  CAS  PubMed  Google Scholar 

  18. Zanolari, B., Friant, S., Funato, K., Sutterlin, C., Stevenson, B.J., and Riezman, H., EMBO J., 2000, vol. 19, no. 12, pp. 2824–2833.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Malinska, K., Malinsky, J., Opekarova, M., and Tanner, W., Mol. Biol. Cell, 2003, vol. 14, no. 11, pp. 4427–4436.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Golubev, V.I., Kulakovskaya, T.V., and Golubeva, E.V., Microbiology (Moscow), 2001, vol. 70, no. 5, pp. 553–556.

    Article  CAS  Google Scholar 

  21. Andrews, J.M., J. Antimicrob. Chemother., 2001, vol. 48, no. 1, pp. 5–16.

    Article  CAS  PubMed  Google Scholar 

  22. Kulakovskaya, E.V., Baskunov, B.P., and Zvonarev, A.N., J. Oleo Sci., 2014, vol. 63, no. 7, pp. 701–707.

    Article  CAS  PubMed  Google Scholar 

  23. Lewis, T., Nichols, P.D., and McMeekin, T.A., J. Microbiol. Methods, 2000, vol. 43, no. 2, pp. 107–116.

    Article  CAS  PubMed  Google Scholar 

  24. Arthington-Skaggs, B.A., Jradi, H., Desai, T., and Morrison, C.J., J. Clin. Microbiol., 1999, vol. 37, no. 10, pp. 3332–3337.

    CAS  PubMed  PubMed Central  Google Scholar 

  25. Ng, H.-E., Raj, S.S., Wong, S.H., Tey, D., and Tan, H.-M., Lett. Appl. Microbiol., 2008, vol. 46, no. 1, pp. 113–118.

    Article  CAS  PubMed  Google Scholar 

  26. Iwaki, T., Iefuji, H., Hiraga, Y., Hosomi, A., Morita, T., Giga-Hama, Y., and Takegawa, K., Microbiology, 2008, vol. 154, no. 3, pp. 830–841.

    Article  CAS  PubMed  Google Scholar 

  27. Bhosle, S.R., Sandhya, G., Sonawane, H.B., and Vaidya, J.G., Int. J. Pharm. Life Sci. (IJPLS), 2011, vol. 2, no. 7, pp. 916–918.

    CAS  Google Scholar 

  28. Flegelova, H., Chaloupka, R., Novotna, D., Malac, J., Gaskova, D., Sigler, K., and Janderova, B., Folia Microbiol., 2003, vol. 48, no. 6, pp. 761–766.

    Article  CAS  Google Scholar 

  29. Turk, M., Montiel, V., Zigon, D., Plemenitas, A., and Ramos, J., Microbiology, 2007, vol. 153, no. 10, pp. 3586–3592.

    Article  CAS  PubMed  Google Scholar 

  30. Mimee, B., Labbe, C., and Belanger, R.R., Glycobiology, 2009, vol. 19, no. 9, pp. 995–1001.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    E. V. Kulakovskaya & A. A. Mironov

Authors
  1. E. V. Kulakovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. Mironov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. V. Kulakovskaya.

Additional information

Original Russian Text © E.V. Kulakovskaya, A.A. Mironov, 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, Vol. 52, No. 6, pp. 584–589.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kulakovskaya, E.V., Mironov, A.A. Resistance to cellobiose lipids and specific features of lipid composition in yeast. Appl Biochem Microbiol 52, 615–620 (2016). https://doi.org/10.1134/S0003683816060107

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683816060107

Keywords

Search

Navigation