Skip to main content
SpringerLink
Log in

Physicochemical and catalytic properties of NAD+- dependent malate dehydrogenase isoforms from maize mesophyll

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

Malate dehyrogenase isoforms (46- and 70-fold purifications) with specific activities of the 640 and 990 U/mg protein were obtained in an electrophoretically homogeneous state from maize mesophyll. The physicochemical and catalytic properties of these isoforms were studied. The molecular weight and the Michaelis constants were determined; the effect of hydrogen ions on the forward and reverse MDH reaction was studied. The results of SDS-PAGE demonstrated that malate dehydrogenase isoforms have an oligomeric structure comprised of identical subunits. The first isoform with a molecular weight of 126.58 kDa is tetramer, and the second isoform with a molecular weight of 63.3 is dimer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Edwards, J. and Walker, D., Fotosintez S3-i S4-rastenii: mekhanizmy i regulyatsiya (Photosynthesis in C3 and C4 Plants: Mechanisms and Regulation), Mokronosov, A.T. Ed., Moscow: Mir, 1986.

  2. Huchzermeyer, B., Hausmann, N., Paquet-Durant, F., and Koyro, H.-W., Trop. Ecol., 2004, vol. 45, no. 1, pp. 141–150.

    CAS  Google Scholar 

  3. Eprintsev, A.T., Klimova, M.A., Shikhalieva, K.D., Fedorin, D.N., Dzhaber, M.T., and Kompantseva, E.I., Biochemistry (Moscow), 2009, vol. 74, no. 7, pp. 793–799.

    CAS  PubMed  Google Scholar 

  4. Eprintsev, A.T., Falaleeva, M.I., Parfenova, I.V., Lyashchenko, M.S., Kompantseva, E.I., and Tret’yakova, A.Yu., Biol. Bull. (Moscow), 2014, vol. 41, no. 6, pp. 486–492.

    Article  CAS  Google Scholar 

  5. Zarivi, O., Cesare, P., Aimola, P., Ragnelli, A.M., Scirri, C., Cimini, A., Bonfigli, A., Pacioni, G., and Miranda, M., FEMS Microbiol. Letts., 2000, vol. 185, no. 2, pp. 213–219.

    Article  CAS  Google Scholar 

  6. Hoshino, T., Hosokawa, N., Yanai, M., Kumasaka, K., and Kawano, K., Rinsho. Byori, 2001, vol. 49, no. 11, pp. 1157–1161.

    CAS  PubMed  Google Scholar 

  7. Popov, V.N., Eprintces, A.T., and Igamberdiev, A.U., J. Plant Physiol., 2003, vol. 160, no. 8, pp. 851–857.

    Article  CAS  PubMed  Google Scholar 

  8. Eprintsev, A.T., Ivent’ev, A.N., and Popov, V.N., Russ. J. Plant Physiol., 2005, vol. 52, no. 4, pp. 553–558.

    Article  CAS  Google Scholar 

  9. Davis, B.J. and Ornstein, L., A New High Resolution Electrophoresis Method, New York: Society for the Study at the New York Academy of Medicine, 1959.

    Google Scholar 

  10. Maurer, R., Disc-Electrophorese, Theorie und Praxis der diskontinuirlichen Polyacrylamidgel-Electrophorese, Heidelberg: Springer, 1968.

    Google Scholar 

  11. Gaal, O., Medgyesi, G.A., and Vereczkey, L., Electrophoresis in the Separation of Biological Macromolecules, New York: John Wiley, 1980.

    Google Scholar 

  12. Fieldes, M.A., Electrophoresis, 1992, vol. 13, nos. 1–2, pp. 82–86.

    Article  CAS  PubMed  Google Scholar 

  13. Nesterenko, M.V., Tilley, M., and Upton, S.J., J. Biochem. Biol., 1994, vol. 28, no. 3, pp. 239–242.

    CAS  Google Scholar 

  14. Shevchenko, A., Wilm, M., Vorm, O., and Mann, M., Anal. Chem., 1996, vol. 68, pp. 850–858.

    Article  CAS  PubMed  Google Scholar 

  15. Laemmly, U.K., Nature, 1970, vol. 77, no. 4, pp. 680–683.

    Article  Google Scholar 

  16. Chernyshev, G.A. and Starikov, V.N., Veroyatnost’ i statistika v biologii i khimii (Probability and Statistics in Biology and Chemistry), Pushkarenko, V.V., Ed., Voronezh: VGU, 1998.

  17. Lehnindger, A.L., Nelson, D.L., and Cox, M.M., Principles of Biochemistry, New York: W.H. Freeman, 2004.

    Google Scholar 

  18. Minárik, P., Tomášková, N., Kollárová, M., and Antalík, M., Gen. Physiol. Biophys., 2002, vol. 21, pp. 257–265.

    PubMed  Google Scholar 

  19. Pinheiro de Carvalho, M.A.A., Zemlyanuchin, A.A., and Eprintsev, A.T., Physiol. Plant., vol. 85, no. 3, p. 547.

  20. Eprintsev, A.T., Popov, V.N., and Shevchenko, M.Yu., Glioksilatnyi tsikl: universal’nyi mekhanizm adaptatsii (Glyoxylate Cycle: A Universal Mechanism of Adaptation), Moscow: IKTs “Akademkniga,” 2007.

    Google Scholar 

  21. Eprintsev, A.T., Falaleeva, M.I., Grabovich, M.Yu., Parfenova, N.V., Kashirskaya, N.N., and Dubinina, G.A., Mkrobiologiya, 2004, vol. 73, no. 4, pp. 437–442.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Voronezh State Utiversity, Voronezh, 394006, Russia

    A. T. Eprintsev, M. O. Gataullina & M. S. Lyashchenko

Authors
  1. A. T. Eprintsev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. O. Gataullina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. S. Lyashchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. T. Eprintsev.

Additional information

Original Russian Text © A.T. Eprintsev, M.O. Gataullina, M.S. Lyashchenko, 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, Vol. 52, No. 4, pp. 365–369.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Eprintsev, A.T., Gataullina, M.O. & Lyashchenko, M.S. Physicochemical and catalytic properties of NAD+- dependent malate dehydrogenase isoforms from maize mesophyll. Appl Biochem Microbiol 52, 366–370 (2016). https://doi.org/10.1134/S0003683816040049

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683816040049

Keywords

Search

Navigation