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Three-dimensional matrixes of natural and synthetic origin for cell biotechnology

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Abstract

The review describes the classification, analysis, characteristics, and prospects of the application of various types of matrixes, of natural or synthetic origin, for cell biotechnology. Particular attention is paid to three-dimensional structures designed on the basis of extracellular matrix (ECM) components with multipotent mesenchymal stromal cells (MMSCs). In particular, the authors previously obtained cell populations with a phenotype similar to MMSCs from farm animal bone marrow (BM) and adipose tissues (AT). A comparative analysis of the properties and features of the derived cellular populations was performed. It was established that MMSCs isolated from BM and AT were capable of forming fatty, bone, and muscle tissue cells when cultured in induction media in vitro. Therefore, the production of farm animal muscle tissue analogs by myogenesis via three-dimensional MMSC cultivation on a porous matrix is a promising research direction for the needs of regenerative medicine, veterinary medicine, and cell and food biotechnology.

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Abbreviations

ECM:

extracellular matrix

HA:

hyaluronic acid

AT:

adipose tissue

BM:

bone marrow

MMSCs:

multipotent mesenchymal stromal cells

PBS:

phosphate buffered saline

AM:

Antharaea mylitta

BM:

Bombix mori

GelMA:

methacrylated gelatin hydrogel

GMP:

good medical practice

ISCT:

International Society for Cellular Therapy

SDF-1:

stromal cell-derived factor-1

PHA:

polyhydroxyalkanoates

SC-PL:

solvent casting particulate leaching

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  1. All-Russia Research Institute for Experimental Veterinary Medicine, Moscow, 109428, Russia

    I. M. Volkova & D. G. Korovina

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  1. I. M. Volkova
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Original Russian Text © I.M. Volkova, D.G. Korovina, 2015, published in Biotekhnologiya, 2015, No. 2, pp. 8–26.

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Volkova, I.M., Korovina, D.G. Three-dimensional matrixes of natural and synthetic origin for cell biotechnology. Appl Biochem Microbiol 51, 841–856 (2015). https://doi.org/10.1134/S0003683815090082

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