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Use of endoglucanase IV from Trichoderma reesei to enhance the hydrolytic activity of a cellulase complex from the fungus Penicillium verruculosum

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Abstract

The effect of polysaccharide monooxygenase (endoglucanase IV) from the fungus Trichoderma reesei on the hydrolysis of polysaccharide substrates by cellulases secreted by the fungus Penicillium verruculosum has been investigated. Supplementation of the enzyme complex from P. verruculosum by endoglucanase IV from T. reesei has been shown to elevate the efficiency of cellulose hydrolysis by 45%.

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References

  1. Bungay, H.R., Energy: The Biomass Options, New York: Wiley and Sons, 1981, p. 347.

    Google Scholar 

  2. Buckeridge, M.S. and Goldman, G.H., Routes to Cellulosic Ethanol, New York: Springer, 2011, p. 261.

    Book  Google Scholar 

  3. Jorgensen, H., Kristensen, J.B., and Felby, C., Biofuels, Bioprod. Bioref., 2007, vol. 1, no. 1, pp. 119–134.

    Article  Google Scholar 

  4. Gusakov, A.V. and Sinitsyn, A.P., Biofuel, Future Sci., 2012, vol. 4, no. 6, pp. 567–569.

    Google Scholar 

  5. Harris, P.V., Welner, D., McFarland, K.C., Re, E., Poulsen, J.C.N., Brown, K., Salbo, R., Ding, H., Vlasenko, E., Merino, S., Xu, F., Cherry, J., Larsen, S., and Leggio, L.L., Biochemistry, 2010, vol. 49, no. 15, pp. 3305–3316.

    Article  CAS  PubMed  Google Scholar 

  6. Koseki, T., Mese, Y., Fushinobu, S., Masaki, K., Fujii, T., Ito, K., Shiono, Y., Murayama, T., and Iefuji, H., Appl. Microbiol. Biotechnol., 2008, vol. 77, no. 6, pp. 1279–1285.

    Article  CAS  PubMed  Google Scholar 

  7. Saloheimo, M., Nakari-Setala, T., Tenkanen, M., and Penttila, M., Eur. J. Biochem., 1997, vol. 249, no. 2, pp. 584–591.

    Article  CAS  PubMed  Google Scholar 

  8. Karlsson, J., Saloheimo, M., Siika-aho, M., Tenkanen, M., Penttila, M., and Tjerneld, F., Eur. J. Biochem., 2001, vol. 268, no. 24, pp. 6498–6507.

    Article  CAS  PubMed  Google Scholar 

  9. Karkehabadi, S., Hansson, H., Kim, S., Piens, K., Mitchinson, C., and Sandgren, M., J. Mol. Biol., 2008, vol. 383, no. 1, pp. 144–154.

    Article  CAS  PubMed  Google Scholar 

  10. Cannella, D., Hsieh, Ch.C., Felby, C., and Jorgensen, H., Biotechnol. Biofuels, 2012, vol. 5, no. 26, pp. 1–10.

    Google Scholar 

  11. Kittl, R., Kracher, D., Burgstaller, D., Haltrich, D., and Ludwig, R., Biotechnol. Biofuels, 2012, vol. 5, no. 1, pp. 1–14.

    Article  Google Scholar 

  12. Beeson, W.T., Phillips, C.M., Cate, J.H.D., and Marletta, M.A., JAC, vol. 134, no. 1, p. 890.

  13. Levasseur, A., Drula, E., Lombard, V., Coutinho, P., and Henrissat, B., Biotechnol. Biofuels, 2013, vol. 6, no. 41, pp. 1–14.

    Google Scholar 

  14. Horn, S.J., Vaaje-Kolstad, G., Westereng, B., and Eijsink, V.G.H., Biotechnol. Biofuels, 2012, vol. 5, no. 1, pp. 45–52.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Gusakov, A.V., Trends Biotechnol., 2011, vol. 29, no. 9, pp. 419–425.

    Article  CAS  PubMed  Google Scholar 

  16. Chekushina, A.V., Dotsenko, G.S., and Sinitsyn, A.P., Kataliz Promyshl., 2012, no. 6, pp. 68–76.

    Google Scholar 

  17. Sinitsyn, A.P., Chernoglazov, V.M., and Gusakov, A.V., Metody issledovaniya i svoistva tsellyuloliticheskikh fermentov (Methods of Study and Properties of Cellulolytic Enzymes), Moscow: VINITI, 1990.

    Google Scholar 

  18. Bissvanger, Kh., Prakticheskaya enzimologiya (A Practical Course in Enzymology), Moscow: BINOM. Laboratoriya znanii, 2010.

    Google Scholar 

  19. Korotkova, O.G., Semenova, M.V., Morozova, V.V., Zorov, I.N., Sokolova, L.M., Bubnova, T.M., Okunev, O.N., and Sinitsyn, A.P., Biokhimiya, 2009, vol. 74, no. 5, pp. 699–709.

    Google Scholar 

  20. Zorov, I.N., Gusakov, A.V., Baraznenok, V.A., Bekkarevich, A.O., Okunev, O.N., Sinitsyn, A.P., and Kondrat’eva, E.G., Appl. Biochem. Microbiol., 2001, vol. 37, no. 6, pp. 587–592.

    Article  CAS  Google Scholar 

  21. Henriksson, G., Johansson, G., and Petterson, J., J. Biotechnol., 2000, vol. 78, no. 1, pp. 93–113.

    Article  CAS  PubMed  Google Scholar 

  22. Sinitsyn, A.P., Osipov, D.O., Rozhkova, A.M., Bushina, E.V., Dotsenko, G.S., Sinitsyna, O.A., Kondrat’eva, E.G., Zorov, I.N., Okunev, O.N., Nemashkalov, V.A., Matys, V.Yu., and Koshelev, A.V., Appl. Biochem. Microbiol., 2014, vol. 50, no. 8, pp. 761–772.

    Article  CAS  Google Scholar 

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Correspondence to O. V. Proskurina.

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Original Russian Text © O.V. Proskurina, O.G. Korotkova, A.M. Rozhkova, E.G. Kondrat’eva, V.Yu. Matys, I.N. Zorov, A.V. Koshelev, O.N. Okunev, V.A. Nemashkalov, T.V. Bubnova, A.P. Sinitsyn, 2015, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2015, Vol. 51, No. 6, pp. 592–599.

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Proskurina, O.V., Korotkova, O.G., Rozhkova, A.M. et al. Use of endoglucanase IV from Trichoderma reesei to enhance the hydrolytic activity of a cellulase complex from the fungus Penicillium verruculosum . Appl Biochem Microbiol 51, 667–673 (2015). https://doi.org/10.1134/S0003683815060125

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  • DOI: https://doi.org/10.1134/S0003683815060125

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