Applied Biochemistry and Microbiology

, Volume 50, Issue 6, pp 601–608 | Cite as

Evaluation of the flocculation potential and characterization of bioflocculant produced by Micrococcus sp. Leo

  • K. OkaiyetoEmail author
  • U. U. Nwodo
  • L. V. Mabinya
  • A. I. Okoh


Bioflocculants are safe, biodegradable and environmentally friendly biopolymeric materials. These merits portend it as preferred alternative to inorganic and organic synthetic polymeric flocculants. The culture conditions optimal for the production of bioflocculant by Micrococcus sp. Leo with subsequent evaluation of the properties of the produced compound were investigated. Optimum culture conditions for bioflocculant production included 2% (vol/vol) inoculum size, incubation temperature of 28°C, agitation speed of 160 rpm and initial pH of 4.0. Glucose and (NH4)2SO4 and Al3+ were the best as sole carbon, nitrogen and cation sources, respectively. The purified bioflocculant flocculated kaolin suspension optimally at a dosage of 0.2 mg/mL following jar test, and flocculating activity of about 70% was retained after heat treatment of 100°C. Chemical analysis showed that the bioflocculant was composed of 28.4% polysaccharide, 2.6% protein and 9.7%. uronic acid. Thermogravimetric analysis demonstrated that the bioflocculant could not decompose completely at 400°C. FTIR spectra revealed the presence of hydroxyl, carboxyl and amino groups as the main functional groups. The bioflocculant produced by Micrococcus sp. Leo appears to hold promise as an alternative to conventional flocculants commonly used in water/wastewater treatment.


Apply Biochemistry Nitrogen Source Agitation Speed Uronic Acid Inoculum Size 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Pleiades Publishing, Inc. 2014

Authors and Affiliations

  • K. Okaiyeto
    • 1
    Email author
  • U. U. Nwodo
    • 1
  • L. V. Mabinya
    • 1
  • A. I. Okoh
    • 1
  1. 1.Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and MicrobiologyUniversity of Fort HareAliceSouth Africa

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