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Bacteria that degrade low-molecular linear epsilon-caprolactam oligomers

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Abstract

Five bacterial strains with the unique ability to utilize low-molecular linear caprolactam oligomers (nylon oligomers) were isolated from soil samples contaminated with industrial wastes of epsilon-caprolactam. Based on the properties studied and also on the analysis of 16S rRNA gene nucleotide sequences, the strains BS2, BS3, BS9, BS38, and BS57 were identified as belonging to the genera Arthrobacter, Brevibacterium, Microbacterium, Gulosibacter, and Achromobacter, respectively. All of the strains also utilized 6-aminohexanoic and adipic acids, which are intermediates of the epsilon-caprolactam catabolism. This indirectly points to the fact that degradation of oligomers in these bacteria occurs via the monomer degradation pathway. The strains BS9 and BS57 utilized only caprolactam oligomers, while BS2, BS3, and BS38 also degraded epsilon-caprolactam and its homologs, enantolactam and caprylolactam, which differentiates the latter from the previously known oligomers-degrading bacteria and suggests the presence in these strains of enzymes with lactam hydrolase activity, in addition to 6-aminohexanoate-dimer hydrolase.

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Authors and Affiliations

  1. Institute of the Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia

    T. Z. Esikova

  2. Tula State University, Tula, 300012, Russia

    E. V. Akatova

  3. “IBC Generium” LLC, Moscow, 127006, Russia

    S. A. Taran

Authors
  1. T. Z. Esikova
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  2. E. V. Akatova
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  3. S. A. Taran
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Corresponding author

Correspondence to T. Z. Esikova.

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Original Russian Text © T.Z. Esikova, E.V. Akatova, S.A. Taran, 2014, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 5, pp. 481–489.

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Esikova, T.Z., Akatova, E.V. & Taran, S.A. Bacteria that degrade low-molecular linear epsilon-caprolactam oligomers. Appl Biochem Microbiol 50, 463–470 (2014). https://doi.org/10.1134/S0003683814050044

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  • DOI: https://doi.org/10.1134/S0003683814050044

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