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Bioflocculant production by Bacillus sp. Gilbert isolated from a marine environment in South Africa

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Abstract

In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa. In the current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail. The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid. The crude bioflocculant retained 44.2% residual flocculating activity after heating at 100°C for 15 min. Chemical analysis of the Bacillus sp. Gilbert purified bioflocculant demonstrated that it was composed mainly of polysaccharide. Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12:7.40:39.92:3.00:13.91. Scanning electron micrograph image of the bioflocculant revealed an amorphous compound.

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References

  1. Salehizadeh, H. and Shojaosadati, S.A., Biotechnol. Adv., 2001, vol. 19, no. 5, pp. 371–385.

    Article  CAS  PubMed  Google Scholar 

  2. Yokoi, H., Arima, T., Hayashi, S., and Takasaki, Y., J. Ferment. Bioeng., 1996, vol. 82, no. 1, pp. 84–87.

    Article  CAS  Google Scholar 

  3. Liu, W., Wang, K., Li, B., Yuan, H., and Yang J., Bioresour. Technol., 2010, vol. 101, no. 3, 1044–1048.

    Article  CAS  PubMed  Google Scholar 

  4. Li, Z., Zhong, S., Lei, H., Chen, R., Yu, Q., and Li, H.L., Bioresour. Technol., 2009, vol. 100, no. 14, pp. 3650–3656.

    Article  CAS  PubMed  Google Scholar 

  5. Dearfield, K.L. and Abermathy, C.O., Mutat. Res., 1988, vol. 195, no. 1, pp. 45–77.

    Article  CAS  PubMed  Google Scholar 

  6. Feng, D.L. and Xu, S.H., World J. Microbiol. Biotechnol., 2008, vol. 24, no. 9, pp. 1627–1632.

    Article  CAS  Google Scholar 

  7. Zheng, Y., Zhi-Long, Y., Xu-Liang, F., Ya-Hong, L., and Wei-Min, C., Bioresour. Technol., 2008, vol. 99, no. 16, pp. 7686–7691.

    Article  CAS  PubMed  Google Scholar 

  8. Xiong, Y., Yuanpeng, W., Yi, Y., Qingbiao, L., Haitao, W., Ronghui, C., and Ning, H., Appl. Environ. Microbiol., 2010, vol. 76, no. 9, pp. 2778–2782.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Elkady, M.F., Soha, F., Sahar, Z., Gadallah, A., and Desouky, A., Bioresour. Technol., 2011, vol. 102, no. 17, pp. 8143–8151.

    Article  CAS  PubMed  Google Scholar 

  10. Berdy, J., J. Antibiot., 2005, vol. 58, no. 1, pp. 1–26.

    Article  CAS  PubMed  Google Scholar 

  11. Piyo, N., Cosa, S., Mabinya, V.L., and Okoh, I.A., Mar. Drugs, 2011, vol. 9, no. 7, pp. 1232–1242.

    Google Scholar 

  12. Zhang, Z., Lin, B., Xia, S., Wang, X., and Yang, A., J. Environ. Sci., 2007, vol. 19, no. 6, pp. 667–673.

    Article  Google Scholar 

  13. Kurane, R., Takeda, K., and Suzuki, T., Agric. Biol. Chem., 1986, vol. 50, no. 9, pp. 2301–2307.

    Article  CAS  Google Scholar 

  14. Cosa, S., Mabinya, L.V., Olaniran, A.O., Okoh, O.O., Bernard, K., Deyzel, S., and Okoh, A.I., Molecules, 2011, vol. 16, no. 3, pp. 2431–2442.

    Article  CAS  PubMed  Google Scholar 

  15. Mabinya, L.V., Cosa, S., Mkwetshana, N., and Okoh, A.I., Molecules, 2011, vol. 16, no. 6, pp. 4358–4370.

    Article  CAS  PubMed  Google Scholar 

  16. Wang, S., Gong, W., Liu, X., Tian, L., Yue, Q., and Gao, B., Biochem. Eng. J., 2007, vol. 36, no. 2, pp. 81–86.

    Article  CAS  Google Scholar 

  17. Gong, W., Wang, S., Sun, X., Liu, X., Yue, Q., and Gao, B., Bioresour. Technol., 2008, vol. 99, no. 11, pp. 4668–4674.

    Article  CAS  PubMed  Google Scholar 

  18. Chang, W.C., Soon, A.Y., In, H.O., and Sang, H.P., Biotechnol. Lett., 1998, vol. 20, no. 12, pp. 643–646.

    Article  Google Scholar 

  19. Chen, H., Zhang, J.F., Jiang, P.J., Yang, S.L., and Liu, Z.L., Environ. Chem., 2002, vol. 21, pp. 360–364.

    Google Scholar 

  20. Gao, J., Bao, H., Xin, M., Liu, Y., Li, Q., and Zhang, Y., J. Zhejiang Univ. Sci. B., 2006, vol. 7, no. 3, pp. 186–192.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F., Anal. Chem., 1956, vol. 28, no. 3, pp. 350–356.

    Article  CAS  Google Scholar 

  22. Jayaraman, J., Laboratory Manual in Biochemistry, Delhi, India: Wiley Eastern, 1981, pp. 75–76.

    Google Scholar 

  23. Ugbenyen, A., Cosa, S., Mabinya, L., Babalola, O.O., Aghdasi, F., and Okoh, A., Int. J. Environ. Res. Public Health, 2012, vol. 9, no. 6, pp. 2108–2120.

    Article  CAS  PubMed  Google Scholar 

  24. Jang, J.H., Ike, M., Kim, S.M., and Fujita, M., Biotechnol. Lett., 2001, vol. 23, no. 8, pp. 593–597.

    Article  CAS  Google Scholar 

  25. Kallos, M.S. and Behie, L.A., Biotechnol. Bioeng., 1999, vol. 63, no. 4, pp. 473–483.

    Article  CAS  PubMed  Google Scholar 

  26. Zhong, J.J. and Yoshida, T., Enzyme Microb. Technol., 1995, vol. 17, no. 12, pp. 1073–1079.

    Article  CAS  Google Scholar 

  27. Xia, S., Zhang, Z., Wang, X., Yang, A., Chen, L., Zhao, J., Leonard, D., and Jaffrezic-Renault, N., Bioresour. Technol., 2008, vol. 99, no. 14, pp. 6520–6527.

    Article  CAS  PubMed  Google Scholar 

  28. Sheng, Y., Zhang, Q., Sheng, Y., Li, C., and Wang, H., Sci. Technol., 2006, vol. 13, pp. 289–292.

    CAS  Google Scholar 

  29. Zhang, J., Liu, Z., Wang, S., and Jiang, P., Appl. Microbiol. Biotechnol., 2002, vol. 59, no. 4–5, pp. 517–522.

    CAS  PubMed  Google Scholar 

  30. Esser, K. and Kues, U., Process Biochem., 1983, vol. 18, no. 1, pp. 21–23.

    CAS  Google Scholar 

  31. Nakamura, J., Miyashiro, S., and Hirose, Y., Agric. Biol. Chem., 1976, vol. 40, pp. 619–24.

    Article  CAS  Google Scholar 

  32. Tago, Y. and Aida, K.O., Appl. Environ. Microbiol., 1977, vol. 34, no. 3, pp. 308–314.

    CAS  PubMed Central  PubMed  Google Scholar 

  33. Salehizadeh, H. and Shojaosadati, S.A., Biotechnol. Lett., 2002, vol. 24, no. 1, pp. 35–40.

    Article  CAS  Google Scholar 

  34. Salehizadeh, H., Vossoughi, M., and Alemzadeh, I., Biochem. Eng. J., 2000, vol. 5, no. 1, pp. 39–44.

    Article  CAS  Google Scholar 

  35. Drugs and the Pharmaceutical Sciences, New York, USA: Marcel Dekker, 1986, vol. 28, pp. 91–125.

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Authors and Affiliations

  1. Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice, 5700, South Africa

    A. M. Ugbenyen, S. Cosa, L. V. Mabinya & A. I. Okoh

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  1. A. M. Ugbenyen
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  2. S. Cosa
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  3. L. V. Mabinya
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  4. A. I. Okoh
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Correspondence to A. M. Ugbenyen.

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Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 1, pp. 59–64.

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Ugbenyen, A.M., Cosa, S., Mabinya, L.V. et al. Bioflocculant production by Bacillus sp. Gilbert isolated from a marine environment in South Africa. Appl Biochem Microbiol 50, 49–54 (2014). https://doi.org/10.1134/S0003683814010104

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  • DOI: https://doi.org/10.1134/S0003683814010104

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