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Expression of eukaryotic recombinant proteins and deriving them from the milk of transgenic animals


Expression in milk of transgenic farm animals is one of the most effective ways to produce eukaryotic recombinant proteins. In this review, we discuss the problems of the appropriateness of selecting this expression system for the production of proteins and the development characteristics of appropriate production processes.

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genetic engineering construct


granulocyte colony-stimulating factor


monoclonal antibodies


recombinant protein


ethylenediamine tetraacetic acid

CaP technology:

calcium phosphate technology


Chinese hamster ovary cell line


Food and Drug Administration (United States)


Good Agricultural Practice


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Correspondence to Yu. M. Khodarovich.

Additional information

Original Russian Text © Yu.M. Khodarovich, I.L. Goldman, E.R. Sadchikova, P.G. Georgiev, 2012, published in Biotekhnologiya, 2012, No. 6, pp. 8–22.

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Khodarovich, Y.M., Goldman, I.L., Sadchikova, E.R. et al. Expression of eukaryotic recombinant proteins and deriving them from the milk of transgenic animals. Appl Biochem Microbiol 49, 711–722 (2013).

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  • biotechnology
  • milk
  • recombinant proteins
  • transgenic animals