Expression of eukaryotic recombinant proteins and deriving them from the milk of transgenic animals

Abstract

Expression in milk of transgenic farm animals is one of the most effective ways to produce eukaryotic recombinant proteins. In this review, we discuss the problems of the appropriateness of selecting this expression system for the production of proteins and the development characteristics of appropriate production processes.

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Abbreviations

GEC:

genetic engineering construct

G-CSF:

granulocyte colony-stimulating factor

monaAB:

monoclonal antibodies

RP:

recombinant protein

EDTA:

ethylenediamine tetraacetic acid

CaP technology:

calcium phosphate technology

CHO:

Chinese hamster ovary cell line

FDA:

Food and Drug Administration (United States)

GAP:

Good Agricultural Practice

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Correspondence to Yu. M. Khodarovich.

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Original Russian Text © Yu.M. Khodarovich, I.L. Goldman, E.R. Sadchikova, P.G. Georgiev, 2012, published in Biotekhnologiya, 2012, No. 6, pp. 8–22.

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Khodarovich, Y.M., Goldman, I.L., Sadchikova, E.R. et al. Expression of eukaryotic recombinant proteins and deriving them from the milk of transgenic animals. Appl Biochem Microbiol 49, 711–722 (2013). https://doi.org/10.1134/S0003683813090020

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Keywords

  • biotechnology
  • milk
  • recombinant proteins
  • transgenic animals