Applied Biochemistry and Microbiology

, Volume 49, Issue 9, pp 711–722 | Cite as

Expression of eukaryotic recombinant proteins and deriving them from the milk of transgenic animals

  • Yu. M. Khodarovich
  • I. L. Goldman
  • E. R. Sadchikova
  • P. G. Georgiev
Problems and Prospects
  • 211 Downloads

Abstract

Expression in milk of transgenic farm animals is one of the most effective ways to produce eukaryotic recombinant proteins. In this review, we discuss the problems of the appropriateness of selecting this expression system for the production of proteins and the development characteristics of appropriate production processes.

Keywords

biotechnology milk recombinant proteins transgenic animals 

Abbreviations

GEC

genetic engineering construct

G-CSF

granulocyte colony-stimulating factor

monaAB

monoclonal antibodies

RP

recombinant protein

EDTA

ethylenediamine tetraacetic acid

CaP technology

calcium phosphate technology

CHO

Chinese hamster ovary cell line

FDA

Food and Drug Administration (United States)

GAP

Good Agricultural Practice

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References

  1. 1.
  2. 2.
    Farid, S.S., J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2007, vol. 848, no. 1, pp. 8–18.PubMedCrossRefGoogle Scholar
  3. 3.
  4. 4.
  5. 5.
    Mazdeh, M., Afzali, S., Jaafari, M.R., Acta Med. Iran., 2010, vol. 48, no. 2, pp. 83–88.PubMedGoogle Scholar
  6. 6.
  7. 7.
    Smyth, S., Khachatourians, G.G., and Phillips, P.W., Nat. Biotechnol., 2002, vol. 20, no. 6, pp. 537–541.PubMedCrossRefGoogle Scholar
  8. 8.
    Harrison, R.L. and Jarvis, D.L., Adv. Virus Res., 2006, vol. 68, pp. 159–191.PubMedCrossRefGoogle Scholar
  9. 9.
    Terra, V.S., Mills, D.C., Yates, L.E., Abouelhadid, S., Cuccui, J., and Wren, B.W., J. Med. Microbiol., 2012, vol. 61, no. 7, pp. 919–926.PubMedCrossRefGoogle Scholar
  10. 10.
    Castilho, A., Neumann, L., Daskalova, S., Mason, H.S., Steinkellner, H., Altmann, F., and Strasser, R., J. Biol. Chem., 2012, vol. 287, no. 43, pp. 36518–36526.PubMedCrossRefGoogle Scholar
  11. 11.
    Echelard, Y., Williams, J.L., Destrempes, M.M., Koster, J.A., Overton, S.A., Pollock, D.P., Rapiejko, K.T., Behboodi, E., Masiello, N.C., Gavin, W.G., Pommer, J., van Patten S.M, Faber, D.C., Cibelli, J.B., and Meade, H.M., Transgenic Res., 2009, vol. 18, no. 3, pp. 361–376.PubMedCrossRefGoogle Scholar
  12. 12.
    Giraldo, P., Rival-Gervier, S., Houdebine, L.M., and Montoliu, L., Transgenic Res., 2003, vol. 12, pp. 751–755.PubMedCrossRefGoogle Scholar
  13. 13.
    Goldman, I.L., Georgieva, S.G., Gurskiy, Y.G., Krasnov, C.A., Deykin, C.A., Popov, A.N., Ermolkevich, T.G., Budzevich, A.I., Chernousov, A.D., and Sadchikova, C.I., Biochem. Cell Biol., 2012, vol. 90, no. 3, pp. 513–519.PubMedCrossRefGoogle Scholar
  14. 14.
  15. 15.
  16. 16.
    Pollock, D.P., Kutzko, J.P., Birck-Wilson, E., Williams, J.L., Echelard, Y., and Meade, H.M., J. Immunol. Methods, 1999, vol. 231, pp. 147–157.PubMedCrossRefGoogle Scholar
  17. 17.
    Zhou, Q., Kyazike, J., Echelard, Y., Meade, H.M., Higgins, E., Cole, E.S., and Edmunds, T., J. Biotechnol., 2005, vol. 117, pp. 57–72.PubMedCrossRefGoogle Scholar
  18. 18.
    Huang, Y.J., Huang, Y., Baldassarre, H., Wang, B., Lazaris, A., Leduc, M., Bilodeau, A.S., Bellemare, A., Côte, M., Herskovits, P., Touati, M., Turcotte, C., Valeanu, L., Lemée, N., Wilgus, H., Bégin, I., Bhatia, B., Rao, K., Neveu, N., Brochu, E., Pierson, J., Hockley, D.K., Cerasoli, D.M., Lenz, D.E., Karatzas, C.N., and Langermann, S., Proc. Natl. Acad. Sci. USA, 2007, vol. 104, pp. 13603–13608.PubMedCrossRefGoogle Scholar
  19. 19.
    Parker, M.H., Birck-Wilson, E., Allard, G., Masiello, N., Day, M., Murphy, K.P., Paragas, V., Silver, S., and Moody, M.D., Protein Expr. Purif., 2004, vol. 38, pp. 177–183.PubMedCrossRefGoogle Scholar
  20. 20.
    Zhang, J., Li, L., Cai, Y., Xu, X., Chen, J., Wu, Y., Yu, H., Yu, G., Liu, S., Zhang, A., Chen, J., and Cheng, G., Protein Expr. Purif., 2008, vol. 57, pp. 127–135.PubMedCrossRefGoogle Scholar
  21. 21.
    Maga, E.A., Cullor, J.S., Smith, W., Anderson, G.B., and Murray, J.D., Foodborne Pathog. Dis., 2006, vol. 3, pp. 384–392.PubMedCrossRefGoogle Scholar
  22. 22.
    Freitas, V.J., Serova, I.A., Andreeva, L.E., Dvoryanchikov, G.A., Lopes, E.S., E.S., Jr., Teixeira, D.I., Dias, L.P., Avelar, S.R., Moura, R.R., Melo, L.M., Pereira, A.F., Cajazeiras, J.B., Andrade, M.L., Almeida, K.C., Sousa, F.C., Carvalho, A.C., and Serov, O.L., An Acad. Bras. Ciens., 2007, vol. 79, no. 4, pp. 585–592.CrossRefGoogle Scholar
  23. 23.
    Lee, C.S., Lee, D.S., Fang, N.Z., Oh, K.B., Shin, S.T., K. and Lee, K., Reprod. Dev. Biol., 2006, vol. 30, pp. 293–299.Google Scholar
  24. 24.
    Ko, J.H., Lee, C.S., Kim, K.H., Pang, M.G., Koo, J.S., Fang, N., Koo, D.B., Oh, K.B., Youn, W.S., Zheng, G.D., Park, J.S., Kim, S.J., Han, Y.M., Choi, I.Y., Lim, J., Shin, S.T., Jin, S.W., Lee, K.K., and Yoo, O.J., Transgenic Res., 2000, vol. 9, pp. 215–222.PubMedCrossRefGoogle Scholar
  25. 25.
    Huang, S., Zhang, K., Huang, Y., Chen, M., Li, H., Lu, D., Lu, J., Chen, Y., Qiu, X., Xue, J., and Zeng, Y., Chin. Sci. Bull., 1998, vol. 43, pp. 1317–1319.CrossRefGoogle Scholar
  26. 26.
    Gama Sosa, M.A., de Gasperi, R., and Elder, G.A., Brain. Struct. Funct., 2010, vol. 214, nos. 2–3, pp. 91–109.PubMedCrossRefGoogle Scholar
  27. 27.
    Houdebine, L.M., Comp. Immunol. Microbiol. Infect. Dis., 2009, vol. 32, no. 2, pp. 107–121.PubMedCrossRefGoogle Scholar
  28. 28.
    Wolf, E., Schernthaner, W., Zakhartchenko, V., Prelle, K., Stojkovic, M., and Brem, G., Exp. Physiol., 2000, vol. 85, no. 6, pp. 615–625.PubMedCrossRefGoogle Scholar
  29. 29.
    Maga, E.A., Sargent, R.G., Zeng, H., Pati, S., Zarling, D.A., Oppenheim, S.M., Collette, N.M., Moyer, A.L., Conrad-Brink, J.S., Rowe, J.D., Bondurant, R.H., Anderson, G.B., and Murray, J.D., Transgenic Res., 2003, vol. 2, no. 4, pp. 485–496.CrossRefGoogle Scholar
  30. 30.
    Hirabayashi, M., Kato, M., Kaneko, R., Hirabayashi, T., Morita, M., and Hochi, S., Exp. Anim., 2006, vol. 55, no. 2, pp. 131–135.PubMedCrossRefGoogle Scholar
  31. 31.
    Zhao, J., Whyte, J., and Prather, R.S., Cell Tissue Res., 2010, vol. 341, no. 1, pp. 13–21.PubMedCrossRefGoogle Scholar
  32. 32.
    Cho, S.K., Hwang, K.C., Choi, Y.J., Bui, H.T., Nguyen, V.T., Park, C., Kim, J.H., and Kim, J.H., J. Reprod. Dev., 2009, vol. 55, no. 2, pp. 128–136.PubMedCrossRefGoogle Scholar
  33. 33.
    Schhieke, A.E., Kind, A.J., Ritchie, W.A., Mycock, K., Scott, A.R., Ritchie, M., Wilmut, I., Colman, A., and Campbell, K.H., Science, 1997, vol. 278, pp. 2130–2133.CrossRefGoogle Scholar
  34. 34.
    Cibelli, J.B., Stice, S.L., Golueke, P.J., Kane, J.J., Jerry, J., Blackwell, C., Ponce de Leon, F.A., and Robi, J.M., Science, 1998, vol. 280, pp. 1256–1258.PubMedCrossRefGoogle Scholar
  35. 35.
    Zhang, Y., Yang, Z., Yang, Y., Wang, S., Shi, L., Xie, W., Sun, K., Zou, K., Wang, L., Xiong, J., Xiang, J., and Wu, J., J. Mol. Cell Biol., 2011, vol. 3, pp. 132–141.PubMedCrossRefGoogle Scholar
  36. 36.
    Honaramooz, A. and Yang, Y., Vet. Med. Int., 2010, pii:657860.Google Scholar
  37. 37.
    Garrels, W., Mátés, L., Holler, S., Dalda, A., Taylor, U., Petersen, B., Niemann, H., Izsvák, Z., Ivics, Z., and Kues, W.A., PLos One, 2011, vol. 6, no. 8, p. 23573.CrossRefGoogle Scholar
  38. 38.
    Schmidt, S., Nat. Biotechnol., 2006, vol. 24, no. 8, p. 877.PubMedCrossRefGoogle Scholar
  39. 39.
  40. 40.
    Antonsen, K.P. and Lee, V.W., US Patent No. 6194553, C07K 14/81, 2001.Google Scholar
  41. 41.
    McCreath, G. and Udell, M., EI Patent No. 1115745, C07K 14/75, 2001.Google Scholar
  42. 42.
    Kutzko, J.P., Hayes, M.L., and Sherman, L.T., US Patent No. 6268487, A23J 1/00, p. 2001.Google Scholar
  43. 43.
  44. 44.
  45. 45.
    Zhang, N., Liu, L., Dumitru, C.D., Cummings, N.R., Cukan, M., Jiang, Y., Li, Y., Li, F., Mithell, T., Mallem, M.R., Ou, Y., Patel, R.N., Vo, K., Wang, H., Burnina, I., Choi, B.K., Huber, H.E., Stadheim, T.A., and Zha, D., MAbs, 2011, vol. 3, no. 3, pp. 289–298.PubMedCrossRefGoogle Scholar
  46. 46.
    Shields, R.L., Lai, J., Keck, R., O’Connell, L.Y., Hong, K., Meng, Y.G., Weikert, S.H., and Presta, L.G., J. Biol. Chem., 2002, vol. 277, no. 30, pp. 26733–26740.PubMedCrossRefGoogle Scholar
  47. 47.
    Yamane-Ohnuki, N. and Satoh, M., MAbs, 2009, vol. 1, no. 3, pp. 230–236.PubMedCrossRefGoogle Scholar
  48. 48.
    Baldassarre, H., Hockley, D.K., Lore, M., Brochu, E., Hakier, B., Zhao, X., and Bordignon, V., Transgenic Res., 2008, vol. 17, no. 1, pp. 73–84.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2013

Authors and Affiliations

  • Yu. M. Khodarovich
    • 1
  • I. L. Goldman
    • 2
  • E. R. Sadchikova
    • 2
  • P. G. Georgiev
    • 2
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia

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