Applied Biochemistry and Microbiology

, Volume 49, Issue 9, pp 711–722 | Cite as

Expression of eukaryotic recombinant proteins and deriving them from the milk of transgenic animals

  • Yu. M. KhodarovichEmail author
  • I. L. Goldman
  • E. R. Sadchikova
  • P. G. Georgiev
Problems and Prospects


Expression in milk of transgenic farm animals is one of the most effective ways to produce eukaryotic recombinant proteins. In this review, we discuss the problems of the appropriateness of selecting this expression system for the production of proteins and the development characteristics of appropriate production processes.


biotechnology milk recombinant proteins transgenic animals 



genetic engineering construct


granulocyte colony-stimulating factor


monoclonal antibodies


recombinant protein


ethylenediamine tetraacetic acid

CaP technology

calcium phosphate technology


Chinese hamster ovary cell line


Food and Drug Administration (United States)


Good Agricultural Practice


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Copyright information

© Pleiades Publishing, Inc. 2013

Authors and Affiliations

  • Yu. M. Khodarovich
    • 1
    Email author
  • I. L. Goldman
    • 2
  • E. R. Sadchikova
    • 2
  • P. G. Georgiev
    • 2
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia

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