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Design of a PCR test based on the gyrA gene sequence for the identification of closely related species of the Bacillus subtilis group

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Abstract

A method for the taxonomic identification of seven closely related bacterial species of the Bacillus subtilis group (B. subtilis, B. amyloliquefaciens, B. licheniformis, B. vallismortis, B. atrophaeus, B. sonorensis, and B. mojavensis) using specific primers selected on the basis of the gyrA gene sequences was developed. The effectiveness of this method both for the identification of pure cultures of type strains of this group and for the precise species identification of collection and industrial bacterial strains was demonstrated. The principal possibility of using this method for detecting B. subtilis group bacteria in mixed cultures was shown.

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Abbreviations

LB medium:

Luria-Bertani medium

PCR:

polymerase chain reaction

T:

type strain

EDTA:

ethylenediamine acid

dNTP:

deoxynucleoside triphosphates

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Authors and Affiliations

  1. State Research Institute for Genetics and Selection of Industrial Microorganisms (GosNIIgenetika), Moscow, 117545, Russia

    L. N. Borshchevskaya, A. N. Kalinina & S. P. Sineokii

Authors
  1. L. N. Borshchevskaya
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  2. A. N. Kalinina
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  3. S. P. Sineokii
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Correspondence to L. N. Borshchevskaya.

Additional information

Original Russian Text © L.N. Borshchevskaya, A.N. Kalinina, S.P. Sineokii, 2012, published in Biotekhnologiya, 2012, No. 3, pp. 32–43.

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Borshchevskaya, L.N., Kalinina, A.N. & Sineokii, S.P. Design of a PCR test based on the gyrA gene sequence for the identification of closely related species of the Bacillus subtilis group. Appl Biochem Microbiol 49, 646–655 (2013). https://doi.org/10.1134/S0003683813070028

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  • DOI: https://doi.org/10.1134/S0003683813070028

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