Abstract
The main and side products of hydroxylation by the C. lunata VKPM F-981 mycelium of fourteen Δ4-3-ketosteroids of the estrane, androstane, and pregnane series and six of their Δ5-3β-hydroxy analogues were identified by H1PMR spectroscopy and comparison with standard samples. The obtained experimental data are considered in terms of the triangular model of the enzyme-substrate interaction. The dependence of the direction of hydroxylation of steroid molecules and the orientation of hydroxy groups on the structure of the initial substrate was revealed.
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Original Russian Text © V.A. Andryushina, V.V. Yaderets, T.S. Stytsenko, A.V. Druzhinina, N.E. Voishvillo, 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, Vol. 49, No. 4, pp. 382–390.
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Andryushina, V.A., Yaderets, V.V., Stytsenko, T.S. et al. Effect of the steroid molecule structure on the direction of its hydroxylation by the fungus Curvularia lunata . Appl Biochem Microbiol 49, 386–394 (2013). https://doi.org/10.1134/S0003683813040030
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DOI: https://doi.org/10.1134/S0003683813040030