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Physicochemical properties and antiproliferative activity of recombinant Yersinia pseudotuberculosis L-asparaginase

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Abstract

The physicochemical, catalytic, and antiproliferative activity of a recombinant L-asparaginase from Yersinia pseudotuberculosis (YpA) have been studied. The following results were obtained: the K M value for L-asparagine is 17 ± 0.9 μM, the optimal temperature is 60°C, pH is 8.0, pI is 5.4 ± 0.3, the L-glutaminase activity is no more than 5–6% of the L-asparaginase activity, and the antiproliferative activity on the Fisher L5178y lymphadenosis cell line comprised T/C = 136% (p < 0.001) at a 15% recovery rate. The described characteristic allows one to regard YpA as an antitumor enzyme with biological features similar to the L-asparaginase of E. coli.

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Authors and Affiliations

  1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya ul. 10, Moscow, 119832, Russia

    V. S. Pokrovskii, M. V. Pokrovskaya, S. S. Aleksandrova, D. D. Zhdanov, N. M. Osmel’yanyuk & N. N. Sokolov

  2. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071, Russia

    R. M. Anrianov

  3. Cancer Research Center, Russian Academy of Medical Sciences, Kashirskoe sh. 24, Moscow, 115478, Russia

    E. M. Treshchalina

Authors
  1. V. S. Pokrovskii
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  2. M. V. Pokrovskaya
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  3. S. S. Aleksandrova
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  4. R. M. Anrianov
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  5. D. D. Zhdanov
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  6. N. M. Osmel’yanyuk
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  7. E. M. Treshchalina
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  8. N. N. Sokolov
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Correspondence to V. S. Pokrovskii.

Additional information

Original Russian Text © V.S. Pokrovskii, M.V. Pokrovskaya, S.S. Aleksandrova, R.M. Anrianov, D.D. Zhdanov, N.M. Osmel’yanyuk, E.M. Treshchalina, N.N. Sokolov, 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, Vol. 49, No. 1, pp. 24–28.

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Pokrovskii, V.S., Pokrovskaya, M.V., Aleksandrova, S.S. et al. Physicochemical properties and antiproliferative activity of recombinant Yersinia pseudotuberculosis L-asparaginase. Appl Biochem Microbiol 49, 18–22 (2013). https://doi.org/10.1134/S000368381301016X

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  • DOI: https://doi.org/10.1134/S000368381301016X

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