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Effect of cyclopentene β,β′-triketone disodium salt on the activity of hydrolases and the state of tobacco mosaic virus particles in tobacco leaves

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Abstract

The activity of hydrolases (protease, RNase) in uninfected and tobacco mosaic virus-infected tobacco leaves of the Samsun variety, untreated and treated with disodium salt of 2-acetyl-4-hydroxycarbonyl-methylthio-5-chlorocyclopent-4-en-1,3-dione (DS), was determined. It was shown that treatment of leaves with this compound significantly increased the activity of hydrolases in them compared to untreated leaves. In infected leaves treated with DS one day before infection, along with an increased level of hydrolases, one revealed more viral particles exposed to destructive changes in infected, rather than untreated, leaves. It is assumed that the DS-caused activation of hydrolases promotes the destruction of viral particles and is therefore one of the cell defense mechanisms induced by this compound that prevents the intracellular accumulation of virus.

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Authors and Affiliations

  1. Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690022, Russia

    L. A. Lapshina, A. V. Reunov, V. P. Nagorskaya, O. P. Shestak & V. L. Novikov

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  1. L. A. Lapshina
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  2. A. V. Reunov
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  3. V. P. Nagorskaya
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  4. O. P. Shestak
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  5. V. L. Novikov
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Correspondence to L. A. Lapshina.

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Original Russian Text © L.A. Lapshina, A.V. Reunov, V.P. Nagorskaya, O.P. Shestak, V.L. Novikov, 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, Vol. 49, No. 1, pp. 67–71.

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Lapshina, L.A., Reunov, A.V., Nagorskaya, V.P. et al. Effect of cyclopentene β,β′-triketone disodium salt on the activity of hydrolases and the state of tobacco mosaic virus particles in tobacco leaves. Appl Biochem Microbiol 49, 59–63 (2013). https://doi.org/10.1134/S0003683813010110

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