Abstract
Approaches to the molecular design of antiviral agents based on the principals of adequacy and mimicry to biopolymers have been discussed. The approaches permit for the reproduction of pharmaceutically valuable properties of the polymeric basis (core) and to create novel nongenetic “programs” of bioactivity of macromolecules by means of grafting specific side group combinations to this core. In vivo experiments have shown that the immunostimulating (in particular, interferon-inducing) antiviral potency of nucleic acids (NAs) is a characteristic of carboxylic acid analogues with an NA-type order of furan and anionic component alteration in the polymer chain. Controlled grafting of virus-targeted side groups of the polymeric basis leads to the direct blockage of viral infection in vitro as an additional antiviral effect. In this work, the inhibition of the early penetration stages of human immunodeficiency virus type 1 (HIV-1) in cells has been studied. Modelling of the macromolecule interaction with the most probable target (the virus-cell fusion mediator (an α-helical complex of the N- heptad repeat regions of three HIV-1 envelope glycoprotein gp41 transmembrane molecules) was performed. Computational docking of the experiment in silico resulted in the clarification of the putative mechanisms and parameters for the programming of site-tropic, axial, and belting blockages of the target via the application of an alicyclic-type side group. The discovered opportunities of multipoint axial cobelting blockage of a target opens up new prospects for the prevention/therapy of AIDS, as well as for other viral infections that use similar systems of envelope fusion peptides (of the first and third types) for penetration into cells.
Similar content being viewed by others
References
Levy, J.A., AIDS (Russ. Ed. Int. AIDS Soc., 2009, vol. 2, no. 2, pp. 99–112.
Ivanskaya, N.V. and Trokhimchuk, T.Yu., Biopol. Cell, 2004, vol. 20, no. 3, pp. 171–181.
Nijhuis, M., Van Maarseveen, N.M., and Boucher, C.F.B., Pharmacology, 2009, vol. 189, pp. 299–320.
Johnson, V.A., Brun-Ve’zinet, F., Clotet, B., Gunthard, H.F., Kuritzkes, D.R., Pillay, D., Schapiro, J.M., and Richman, D.D., Top. HIV Med., 2010, vol. 18, no. 5, pp. 156–163.
da Silva, J., Curr. HIV Res., 2003, vol. 1, no. 3, pp. 363–371.
Serbin, A.V., Karaseva, E.N., Tsvetkov, V.B., Alikhanova, O.L., and Rodionov, I.L., Macromol. Symp., 2010, vol. 296, no. 1, pp. 466–477.
Ershov, F.I. and Kiselev, O.I., Interferony i ikh induktory (ot molekul do lekarstv) (Interferons and Their Inducers: From Molecules to Drugs), Moscow: GEOTAR-Media, 2005.
Christopher, M.E. and Wong, J.P., Int. J. Mol. Sci., 2008, vol. 9, pp. 1561–1594.
Kumar, H. and Akira, S., Biochem. Biophys. Res. Commun., 2009, vol. 388, no. 4, pp. 621–625.
Ershov, F.I., Vinograd, I.A., Kozlovskii, M.M., Pentsarskii, O.Ya., and Stoikaya, L.L., Polimery meditsinskogo naznacheniya (Polymers Intended for Medicine), Plate, N.A, Ed., Moscow: Nauka, 1988, pp. 111–126.
Barinsky, I.F., Krentsel, B.A., Gribencha, S.V., Serbin, A.V., Davydova, A.A., Stotskaya, L.L., Lazarenko, A.A., and Berezina, L.K., Sov. Med. Rev. E: Virol. Rev., 1991, no. 4, pp. 79–102.
Ehrlich, P., Uber den Jetzigen Stand der Chemotherapie, Ber. Dtsch. Chem. Ges., 1909, vol. 42, pp. 17–47.
Timofeev, D.I., Perminova, N.G., Serbii, A.V., Kiseleva, Ya.Yu., Neklyudov, V.V., Vatolin, G.Yu., Kas’yan, L.I., Grebinik, T.S., and Timofeev, I.V., Antibiot. Khimioter., 2003, vol. 48, no. 5, pp. 7–15.
Serbin, A.V., Kasyan, L.I., Bourcteine, M.E., and Boukrinskaya, A.G., Antivir. Res., 1999, vol. 41, no. 2, p. 46.
Kiseleva, Ya.Yu., Perminova, N.G., Plyasunova, O.A., Timofeev, D.I., Serbii, A.V., Kas’yan, L.I., Egorov, Yu.A., Grebinik, T.S., and Timofeev, I.V., Mol. Genet. Epidemiol. Virol., 2005, no. 2, pp. 33–36.
Egorov, Y., Serbin, A., Alikhanova, O., Burshtein, M., Lupandin, S., and Bukrinskaya, A., Antivir. Res., 2007, vol. 74, no. 3, p. 49.
Egorov, Y., Serbin, A., Kasyan, L., Tarabara, I., and Alikhanova, O., Antivir. Res., 2006, vol. 70, no. 1, p. 42.
Serbin, A., Karaseva, E., Egorov, Y., Dunaeva, I., Pavlova, M., and Kushch, A., Antivir. Res., 2009, vol. 82, no. 2, p. 66.
Perminova, N.G., Serbii, A.V., Timofeev, D.I., Plyasunova, O.A., Kiseleva, Ya.Yu., Karpyshev, N.N., Vatolin, G.Yu., Neklyudov, V.V., Bakunina, A.Yu., and Timofeev, I.V., Biotekhnologiya, 2003, no. 5, pp. 26–36.
Timofeev, I.V., Perminova, N.G., Serbii, A.V., Timofeev, D.I., and Plyasunova, O.A., RF Patent No. 2315617, 2005.
Serbin, A.V., Timofeyev, I.V., Alikhanova, O.L., Perminova, N.G., Semerikov, V.N., Karpyshev, N.N., Ermakov, I.V., Timofeyev, D.I., Grebinic, T.S., Bakulina, A.Y., Margolis, L., and Kiseleva, Y.Y., Antivir. Res., 2004, vol. 62, no. 2, p. 35.
Karaseva, E., Serbin, A., Rodionov, I., Vasiliev, A., Abramov, V., and Alikhanova, O., Antivir. Res., 2009, vol. 82, no. 2, p. 59.
Serbin, A., Egorov, Y., and Alikhanova, O., Antivir. Res., 2007, vol. 74, no. 3, p. 51.
Burshtein, M.E., Serbin, A.V., Khakhulina, T.V., Alymova, I.V., Stotskaya, L.L., Bogdan, O.P., Manukchina, E.E., Jdanov, V.V., Sharova, N.K., and Bukrinskaya, A.G., Antivir. Res., 1999, vol. 41, no. 3, pp. 135–144.
Timofeev, I.V., Bakunina, A.Yu., Serbii, A.V., Timofeev, D.I., Kiseleva, Ya.Yu., and Perminova, N.G., Biotekhnologiya, 2002, no. 4, pp. 15–31.
Lu, M., Ji, H., and Shen, S., J. Virol., 1999, vol. 73, no. 5, pp. 4433–4438.
Lu, M., Stoller, M.O., Wang, S., Liu, J., Fagan, M.B., and Nunberg, J.H., J. Virol., 2001, vol. 75, no. 22, pp. 11146–11156.
Tan, K., Liu, J.H., Wang, J.H., Shen, S., and Lu, M., Proc. Natl. Acad. Sci. USA, 1997, vol. 94, no. 11, pp. 12303–12308.
Koshiba, T. and Chan, D.C., J. Biol. Chem., 2003, vol. 278, no. 9, pp. 7573–7579.
Eckhardt, M., Doctoral (Nat. Sci.) Dissertation, Heidelberg: Ruperto-Carola Univ., 2010.
Serbin, A., Karaseva, E., Chernikova, E., Dunaeva, I., Krutko, E., Filatova, M., and Zezin, A., Macromol. Symp., 2010, vol. 296, no. 1, pp. 80–91.
Boukrinskaia, A.G., Serbin, A.V., Bogdan, O.P., Stotskaya, L.L., Alymova, I.V., and Klimochkin, Yu.N., US Patent No. 5880154, 1999.
Bukrinskaya, A.G., Burshtein, M.E., and Alikhanova, O.L., RF Patent No. 2281297, 2006.
Gasteiger, J. and Marsili, M., Tetrahedron Lett., 1978, vol. 19, no. 34, pp. 3181–3184.
Stewart, J.J.P., in Encyclopedia of Computational Chemistry, Schleyer, P.V.R., Allinger, N.L., Clark, T., Gasteiger, J., Kollman, P.A., Schaefer, H.F., and Schreiner, P.R., Eds., Chichester: Wiley, 1998, Vol. 3, pp. 2080–2086.
Serbii, A.V., Karaseva, E.N., Fedorova, N.E., Pavlova, M.V., Klimova, E.V., Leont’eva, M.V., and Kushch, A.A., Antibiot. Khimioter., 2007, vol. 52, no. 11–12, pp. 8–13.
Pavlova, M.V., Fedorova, N.E., Serbii, A.V., Egorov, Yu.A., Karaseva, E.N., Klimova, E.V., and Kushch, A.A., Antibiot. Khimioter., 2008, vol. 53, nos. 7–8, pp. 8–14.
Pavlova, M.V., Fedorova, N.E., Serbii, A.V., Klimova, E.V., Karaseva, E.N., Golyshev, S.A., Polyakov, V.Yu., and Kushch, A.A., Antibiot. Khimioter., 2008, vol. 53,part 11–12, pp. 3–10.
Pavlova, M., Serbin, A., Fedorova, N., Karaseva, E., Klimova, E., and Kushch, A., Antivir. Res., 2009, vol. 82, no. 2, pp. 50–51.
Wohl, D.A., Zeng, D., Stewart, P., Glomb, N., Alcorn, T., Jones, S., Handy, J., Fiscus, S., Weinberg, A., Gowda, D., and van der Horst, C., J. AIDS, 2005, vol. 38, no. 5, pp. 538–544.
Chan, D.C., Fass, D., Berger, J.M., and Kim, P.S., Cell, 1997, vol. 89, no. 2, pp. 263–273.
Follis, K.E., Larson, S.J., Lu, M., and Nunberg, J.H., J. Virol., 2002, vol. 76, no. 14, pp. 7356–7362.
Markosyan, K.M., Ma, X., Lu, M., and Nunberg, J.H., Virology, 2002, vol. 302, no. 1, pp. 174–184.
Qadir, M.I. and Malik, S.A., AIDS Res. Hum. Retrovir., 2010, vol. 26, no. 11, pp. 1–7.
Sabin, C., Corti, D., Buzon, V., Seaman, M.S., Hulsik, D.L., Hinzl, A., Vanzetta, F., Agatic, G., Silacci, C., Mainetti, L., Scarlatti, G., Sallusto, F., Weiss, R., Lanzavecchia, A., and Weissenhorn, W., PLoS Pathogens, 2010, vol. 6, no. 11, p. el001195.
Muegge, I. and Martin, Y.C., J. Med. Chem., 1999, vol. 42, no. 5, pp. 791–804.
Kuntz, I.D., Blaney, J.M., Oatley, S.J., Langridge, R., and Ferrin, T.E., J. Mol. Biol., 1982, vol. 161, no. 2, pp. 269–288.
Jones, G., Willett, P., Glen, R., Leach, A.R., and Taylor, R., J. Mol. Biol., 1997, vol. 267, no. 3, pp. 727–748.
Eldridge, M.D., Murray, C.W., Auton, T.R., Paolini, G.V., and Mee, R.P., J. Comp. Aided Molec. Des., 1997, vol. 11, no. 5, pp. 425–445.
Cai, L. and Jiang, S., Chem. Med. Chem., 2010, vol. 5, no. 11, pp. 1813–1824.
Timofeev, D.I., Perminova, N.G., Serbii, A.V., and Timofeev, I.V., Antibiot. Khimioter., 2003, vol. 48, no. 2, pp. 29–41.
Palfreyman, M.T., in Encyclopedia of Life Sciences, Palfreyman, M.T. and Jorgensen, E.M., Eds., Chichester: Wiley, 2009, pp. 1–14.
LeDuc, D.L. and Shin, Y.K., Biosci. Rep., 2000, vol. 20, no. 6, pp. 557–570.
Dutch, R.E., Jardetzky, T.S., and Lamb, R.A., Biosci. Rep., 2000, vol. 20, no. 6, pp. 597–612.
Porotto, M., Doctor, L., Carta, P., Fornabaio, M., Greengard, O., Kellogg, G.E., and Moscona, A., J. Virol., 2006, vol. 80, no. 19, pp. 9837–9849.
Yu, M., Wang, E., Liu, Y., Cao, D., Jin, N., Zhang, C.W.H., Bartlam, M., Rao, Z., Tien, P., and Gao, G.F., J. Gen. Virol., 2002, vol. 83, no. 3, pp. 623–629.
Rahaman, A., Srinivasan, N., Shamala, N., and Shaila, M.S., Biochemistry, 2003, vol. 42, no. 4, pp. 922–931.
Douglas, J.L., Panis, M.L., Ho, E., Lin, K.Y., Krawczyk, S.H., Grant, D.M., Cai, R., Swaminathan, S., Chen, X., and Cihlar, T., Antimicrob. Agents Chemother., 2005, vol. 49, no. 6, pp. 2460–2466.
Masignani, V., Rappuoli, R., Savino, S., and Artco, M.B., US Pat. Appl. No. 20090176699, 2009.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.V. Serbin, A.V. Veselovskii, V.B. Tsvetkov, 2012, published in Biotekhnologiya, 2012, No. 1, pp. 72–89.
Rights and permissions
About this article
Cite this article
Serbin, A.V., Veselovskii, A.V. & Tsvetkov, V.B. In vitro and in silico investigation of interferonogenic analogues of nucleic acids, artificially programmed to block the initial stages of HIV infection of cells. Appl Biochem Microbiol 48, 723–739 (2012). https://doi.org/10.1134/S0003683812090049
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683812090049