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Oxidation of sulfur-containing substrates by aboriginal and experimentally designed microbial communities

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Abstract

Aboriginal and experimental (constructed of pure microbial cultures) communities of acidophilic chemolithotrophs have been studied. The oxidation of elemental sulfur, sodium thiosulfate, and potassium tetrathionate as sole sources of energy has been monitored. The oxidation rate of the experimental community is higher as compared to the aboriginal community isolated from a flotation concentrate of pyrrhotine-containing pyrite-arsenopyrite gold-arsenic sulfide ore. The degree of oxidation of the mentioned S substrates amounts to 17.91, 68.30, and 93.94% for the experimental microbial community and to 10.71, 56.03, and 79.50% for the aboriginal community, respectively. The degree of oxidation of sulfur sulfide forms in the ore flotation concentrate is 59.15% by the aboriginal microbial community and 49.40% by the experimental microbial community. Despite a higher rate of oxidation of S substrates as a sole source of energy by the experimental microbial community, the aboriginal community oxidizes S substrates at a higher rate in the flotation concentrate of pyrrhotine-containing pyrite-arsenopyrite gold-arsenic sulfide ore, from which it was isolated. Bacterial-chemical oxidation of the flotation concentrate by the aboriginal microbial community allows for the extraction of an additional 32.3% of gold from sulfide minerals, which is by 5.7% larger compared to the yield obtained by the experimental microbial community.

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References

  1. Kondrat’eva, T.F., Pivovarova, T.A., Tsaplina, I.A., Fomchenko, N.V., Zhuravleva, A.E., Murav’ev, M.I, Melamud, V.S., and Bulaev, A.G., Microbiology, 2012, vol. 81, no. 1, pp. 3–28.

    PubMed  Google Scholar 

  2. Shippers, A. and Sand, W., Appl. Environ. Microbiol., 1999, vol. 65, no. 1, pp. 319–321.

    Google Scholar 

  3. Shippers, A., Jozsa, P.G., and Sand, W., Appl. Environ. Microbiol., 1996, vol. 62, no. 9, pp. 3424–3431.

    Google Scholar 

  4. Sand, W., Gehrke, T., Jozsa, P.-G., and Schippers, A., Hydrometallurgy, 2001, vol. 59, nos. 2–3, pp. 159–175.

    Article  CAS  Google Scholar 

  5. van Aswegen, P.C., van Niekerk, J., and Olivier, W., in Biomining, Rawlings, D.E., Johnson, B.D., and Eds., Berlin: Springer-Verlag, 2007, pp. 1–35.

    Chapter  Google Scholar 

  6. Luthy, R.G. and Bruce, S.G., Environ. Sci. Technol., 1979, vol. 13, no. 12, pp. 1481–1487.

    Article  CAS  Google Scholar 

  7. Sedelnikova, G.V. and Savari, E.E., in Proc. 15th Int. Biohydrometallurgy Sympos. (IBS 2003). Part I, Tsezos, M., Remoudaki, E., and Hatzikioseyian, A., Athens: Nat. Techn. Univ. Athens, 2003, pp. 91–100.

    Google Scholar 

  8. Thomas, J.E., Jones, C.F., Skinner, W.M., and Smart, RSt.C., Geochim. Cosmochim., 1998, vol. 62, no. 9, pp. 1555–1565.

    Article  CAS  Google Scholar 

  9. Lindstrom, E.B. and Gunneriusson, L., J. Ind. Microbiol., 1990, vol. 5, no. 6, pp. 375–382.

    Article  Google Scholar 

  10. Lindstrom, E.B., Sandstrom, A., and Sundkvist, J.-E., Hydrometallurgy, 2003, vol. 71, nos. 1–2, pp. 21–30.

    Article  CAS  Google Scholar 

  11. Silverman, M.P. and Lundgren, D.C., J. Bacteriol., 1959, vol. 77, no. 5, pp. 642–647.

    PubMed  CAS  Google Scholar 

  12. Bulaev, A.G., Pivovarova, T.A., Melamud, V.S., Bumazhkin, B.K., Patutina, E.O., Kolganova, T.V., Kuznetsov, B.B., and Kondrat’eva, T.F., Microbiology, 2011, vol. 80, no. 6, pp. 854–861.

    Article  Google Scholar 

  13. Kolmert, A., Wikstrom, P., and Hallberg, K.B., J. Microbiol. Methods, 2000, vol. 41, no. 3, pp. 179–184.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, 117312, Russia

    T. A. Pivovarova, A. G. Bulaev, P. V. Roshchupko & T. F. Kondrat’eva

  2. ZAO Polyus, Krasnoyarsk, Russia

    A. V. Belyi

Authors
  1. T. A. Pivovarova
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  2. A. G. Bulaev
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  3. P. V. Roshchupko
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  4. A. V. Belyi
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  5. T. F. Kondrat’eva
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Corresponding author

Correspondence to T. F. Kondrat’eva.

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Original Russian Text © T.A. Pivovarova, A.G. Bulaev, P.V. Roshchupko, A.V. Belyi, T.F. Kondrat’eva, 2012, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2012, Vol. 48, No. 6, pp. 640–645.

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Pivovarova, T.A., Bulaev, A.G., Roshchupko, P.V. et al. Oxidation of sulfur-containing substrates by aboriginal and experimentally designed microbial communities. Appl Biochem Microbiol 48, 577–582 (2012). https://doi.org/10.1134/S0003683812060099

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  • DOI: https://doi.org/10.1134/S0003683812060099

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