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Biodegradation of polyhydroxyalkanoates by soil microbial communities of different structures and detection of PHA degrading microorganisms

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Abstract

Biodegradation of microbial linear polymers of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) by soil microbial communities of different structures has been studied during two field seasons in different weather conditions. This process was shown to be influenced by the polymer chemical composition, temperature, humidity, and the microbial soil component. The PHA degradation was accompanied by a decrease in the polymer molecular weight and an increase in the degree of crystallinity, indicating the preferential destruction of the amorphous phase compared to the crystalline one. The quantity of the true PHA destructors developing at the surface of the polymer samples was lower than the quantity of accompanying bacteria. The dominant PHA degrading microorganisms under the test conditions were identified as bacteria of the genera Variovorax, Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus, and Xanthomonas and as micromycetes from Penicillium, Paecilomyces, Acremonium, Verticillium, and Zygosporium.

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References

  1. Chen, G.-Q., Chem. Soc. Rev., 2009, vol. 38, no. 8, pp. 2434–2446.

    Article  PubMed  CAS  Google Scholar 

  2. Kim, D.Y., Kim, H.W., Chung, M.G., and Rhee, Y.H., J. Microbiol., 2007, vol. 45, no. 2, pp. 87–97.

    PubMed  Google Scholar 

  3. Bonartseva, G.A., Myshkina, V.L., Nikolaeva, D.A., Kevbrina, M.V., Kallistova, A.Y., Gerasin, V.A., Iordanskii, A.L., and Nozhevnikova, A.N., Appl. Biochem. Biotechnol., 2003, vol. 109, nos. 1–3, pp. 285–301.

    Article  PubMed  CAS  Google Scholar 

  4. Mergaert, J., Webb, A., Anderson, C., Wouters, A., and Swings, J., Appl. Environ. Microbiol., 1993, vol. 59, no. 10, pp. 3233–3238.

    PubMed  CAS  Google Scholar 

  5. Suyama, T., Tokiwa, Y., Ouichanpagdee, P., Kanagawa, T., and Kamagata, Y., Appl. Environ. Microbiol., 1998, vol. 64, no. 12, pp. 5008–5011.

    PubMed  CAS  Google Scholar 

  6. Woolnough, C.A., Charlton, T., Yee, L.H., Sarris, M., and Foster, J.R., Polym. Int., 2008, vol. 57, no. 9, pp. 1042–1051.

    Article  CAS  Google Scholar 

  7. Mokeeva, V.L., Chekunova, L.N., Myshkina, V.L., Nikolaeva, D.A., Gerasin, V.A., and Bonartseva, G.A., Mikol. Fitopatol., 2002, vol. 36, no. 5, pp. 59–63.

    CAS  Google Scholar 

  8. Bhatt, R., Shah, D., Patel, K.C., and Trivedi, U., Biores. Technol., 2008, vol. 99, no. 11, pp. 4615–4620.

    Article  CAS  Google Scholar 

  9. Colak, A. and Guner, S., Int. Biodeterior. Biodegrad., 2004, vol. 53, pp. 103–109.

    Article  CAS  Google Scholar 

  10. Nishida, H. and Tokiwa, Y., J. Environ. Polym. Degrad., 1993, vol. 1, no. 1, pp. 65–80.

    Article  CAS  Google Scholar 

  11. Mukai, K. and Doi, Y., RIKEN Review, 1993, no. 3, pp. 21–22.

  12. Lim, S.-P., Gan, S.-N., and Tan, I., Appl. Biochem. Biotechnol., 2005, vol. 126, no. 1, pp. 23–32.

    Article  PubMed  CAS  Google Scholar 

  13. Sridewi, N., Bhubalan, K., and Sudesh, K., Polym. Degrad. Stab., 2006, vol. 91, no. 12, pp. 2931–2940.

    Article  CAS  Google Scholar 

  14. Lopez-Llorca, L.V., Colom Valiente M.F., Gascon A, Micron, 1993, vol. 24, no. 1, pp. 23–29.

    Article  CAS  Google Scholar 

  15. Yew, S.-P., Tang, H.-Y., and Sudesh, K., Polym. Degrad. Stab., 2006, vol. 91, no. 8, pp. 1800–1807.

    Article  CAS  Google Scholar 

  16. Chowdhury, A.A., Arch. Mikrobiol., 1963, vol. 47, pp. 167–200.

    Article  PubMed  CAS  Google Scholar 

  17. Brandl, H. and Puchner, P., Biodegradation, 1991, vol. 2, no. 4, pp. 237–243.

    Article  CAS  Google Scholar 

  18. Briese, B.-H., Jendrossek, D., and Schlegel, H.G., FEMS Microbiol. Letts., 1994, vol. 117, no. 1, pp. 107–112.

    Article  CAS  Google Scholar 

  19. Mergaert, J., Anderson, C., Wouters, A., and Swings, J., J. Environ. Polym. Degrad., 1994, vol. 2, no. 3, pp. 177–183.

    Article  CAS  Google Scholar 

  20. RF Patent No. 2053292, 1996.

  21. Volova, T.G., Kalacheva, G.S., and Steinbuchel, A., J. Sib. Fed. Univ. Biol., 2008, vol. 1, no. 1, pp. 91–101.

    Google Scholar 

  22. Zvyagintsev, D.G., Metody pochvennoi mikrobiologii i biokhimii (Methods of Soil Microbiology and Biochemistry), Moscow: Mosk. Gos. Univ., 1990.

    Google Scholar 

  23. Voinova, O.N, Kalacheva, G.S., Grodnitskaya, I.D., and Volova, T.G., Appl. Biochem. Microbiol., 2009, vol. 45, no. 4, pp. 427–431.

    Article  CAS  Google Scholar 

  24. Bergey’s Manual of Determinative Bacteriology, Holt, J., Krieg, N., Staley, J., and Williams, S., Eds., Baltimore: Williams and Wilkins, 1994. Translated under the title Opredelitel’ bakterii Berdzhi, Moscow: Mir, 1997.

    Google Scholar 

  25. Weyant, S.R., Moss, W., Weaver, R., Hollis, D., Jordan, J., Cook, F., and Daneshvar, M., Opredelitel’ netrivial’nykh patogennykh gramotritsatel’nykh bakterii (Identification of Unusual Pathogenic Gram-Negative Aerobic Bacteria), Moscow: Mir, 1999.

    Google Scholar 

  26. Egorova, L.N., Pochvennye griby Dal’nego Vostoka (Soil Fungi of the Far East), Leningrad: Nauka, 1986.

    Google Scholar 

  27. Sutton, D., Opredelitel’ patogennykh i uslovno patogennykh gribov (Determinant of Pathogenic and Conditionally Pathogenic Fungi), Sutton, D., Fotergill, A., and Rinaldi, M., Eds., Moscow: Mir, 2001.

    Google Scholar 

  28. Kurakov, A.V., Novikova, N.D., Ozerskaya, S.M., Deshevaya, E.A., Gevorkyan, S.A., and Goginyan, V.B., Kosm. Biol. Aviakosm. Med., 2007, vol. 41, no. 5, pp. 49–56.

    CAS  Google Scholar 

  29. Kondratyuk, T.A., Immunopatol. Allergol. Infektol., 2010, no. 1, pp. 65–66.

  30. Delafield, F.P., Doudoroff, M., Palleroni, N.J., and Lusty, C.J., J. Bacteriol., 1965, vol. 90, no. 5, pp. 1455–1466.

    PubMed  CAS  Google Scholar 

  31. Van de Peer, Y. and De Wachter, R., Comput. Applic. Biosci., 1993, vol. 9, no. 2, pp. 177–182.

    Google Scholar 

  32. Volova, T.G., Lukovenko, S.G., and Vasil’ev, A.D., Biotekhnologiya, 1992, no. 1, pp. 19–22.

  33. Rosa, D.S., Filho, R.P., Chui, Q.S.H., and Guedes, C.G.F., Eur. Polym J, 2003, vol. 39, no. 2, pp. 233–237.

    Article  CAS  Google Scholar 

  34. Jendrossek, D. and Handrick, R., Annu. Rev. Microbiol., 2002, vol. 56, pp. 403–432.

    Article  PubMed  CAS  Google Scholar 

  35. Abe, H., Doi, Y., Aoki, H., and Akehata, T., Macromolecules, 1998, vol. 31, no. 7, pp. 1791–1797.

    Article  CAS  Google Scholar 

  36. Volova, T.G., Boyandin, A.N., Vasiliev, A.D., Karpov, V.A., Prudnikova, S.V., Mishukova, O.V., Boyarskikh, U.A., Filipenko, M.L., Rudnev, V.P., Bui Ba Xuan, Vũ Viât Dñg, and Gitelson, I.I., Polym. Degrad. Stab., 2010, vol. 95, no. 12, pp. 2350–2359.

    Article  CAS  Google Scholar 

  37. Quinteros, R., Goodwin, S., Lenz, R.W., and Park, W.H., Int. J. Biol. Macromol., 1999, vol. 25, nos. 1–3, pp. 135–143.

    Article  PubMed  CAS  Google Scholar 

  38. Sang, B.-I., Hori, K., Tanji, Y., and Unno, H., Appl. Microbiol. Biotechnol., 2002, vol. 58, no. 2, pp. 241–247.

    Article  PubMed  CAS  Google Scholar 

  39. Lee, K.-M., Gimore, D.F., and Huss, M.J., J. Polym. Environ., 2005, vol. 13, no. 3, pp. 213–219.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, building 50, Akademgorodok 50, Krasnoyarsk, 660036, Russia

    A. N. Boyandin & T. G. Volova

  2. Siberian Federal University, pr. Svobodny 79, Krasnoyarsk, 660041, Russia

    S. V. Prudnikova

  3. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent’eva 8, Novosibirsk, 630090, Russia

    M. L. Filipenko & E. A. Khrapov

  4. Kirenskii Institute of Physics, Siberian Branch, Russian Academy of Sciences, building 38, Akademgorodok 50, Krasnoyarsk, 660036, Russia

    A. D. Vasil’ev

Authors
  1. A. N. Boyandin
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  2. S. V. Prudnikova
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  3. M. L. Filipenko
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  4. E. A. Khrapov
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  5. A. D. Vasil’ev
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  6. T. G. Volova
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Correspondence to A. N. Boyandin.

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Original Russian Text © A.N. Boyandin, S.V. Prudnikova, M.L. Filipenko, E.A. Khrapov, A.D. Vasil’ev, T.G. Volova, 2012, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2012, Vol. 48, No. 1, pp. 35–44.

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Boyandin, A.N., Prudnikova, S.V., Filipenko, M.L. et al. Biodegradation of polyhydroxyalkanoates by soil microbial communities of different structures and detection of PHA degrading microorganisms. Appl Biochem Microbiol 48, 28–36 (2012). https://doi.org/10.1134/S0003683812010024

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  • DOI: https://doi.org/10.1134/S0003683812010024

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