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Optimization of roller culturing of influenza virus vaccine strains in MDCK and Vero cell cultures using plant origin components

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Abstract

Roller culturing of MDCK and Vero cells in an experimental nutrient medium based on soy flour hydrolysate, plant material obtained using the bromelain plant enzyme, was studied. The medium supplemented with 2 or 3% fetal calf serum (FCS) had a strong growth-stimulating effect on Vero and MDCK and cells, respectively, and did not alter the cell morphology. A/Solomon Islands/03/06 (H1N1) and B/Malaysia/2506/04 influenza vaccine viruses were grown on MDCK and Vero cell cultures obtained as a result of culturing in rollers on media containing soy flour hydrolysate and FCS (2 or 3%, respectively). The titer of the viruses was high in the presence of either trypsin (2 μg/ml) or bromelain (20 μg/ml).

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Abbreviations

WHO:

World Health Organization

PI:

proliferation index

TCC:

transplantable cell culture

HAR:

hemagglutination reaction

DCE:

developing chick embryos

FCS:

fetal calf serum

TCD 50 :

50% tissue cytotoxic dose

EDTA:

ethylenediaminetetraacetic acid

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Authors and Affiliations

  1. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences, ul. Gamalei 16, Moscow, 123098, Russia

    E. I. Isaeva & R. Ya. Podchernyayeva

  2. Vektor State Scientific Center of Virology and Biotechnology, p. Kol’tsovo, Novosibirsk oblast, 630559, Russia

    N. A. Mazurkova, M. O. Skarnovich, G. P. Troshkova & L. N. Shishkina

Authors
  1. E. I. Isaeva
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  2. N. A. Mazurkova
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  3. M. O. Skarnovich
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  4. G. P. Troshkova
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  5. L. N. Shishkina
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  6. R. Ya. Podchernyayeva
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Corresponding author

Correspondence to N. A. Mazurkova.

Additional information

Original Russian Text © E.I. Isaeva, N.A. Mazurkova, M.O. Skarnovich, G.P. Troshkova, L.N. Shishkina, R.Ya. Podchernyayeva, 2010, published in Biotekhnologiya, 2010, No. 6, pp. 55–62.

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Isaeva, E.I., Mazurkova, N.A., Skarnovich, M.O. et al. Optimization of roller culturing of influenza virus vaccine strains in MDCK and Vero cell cultures using plant origin components. Appl Biochem Microbiol 47, 737–743 (2011). https://doi.org/10.1134/S0003683811080059

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  • DOI: https://doi.org/10.1134/S0003683811080059

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