Abstract
Influence of cloned regulatory genes on nogalamycin biosynthesis by Streptomyces nogalater LV65 strain has been studied. Gene snorA from the S. nogalater genome was cloned in multicopy replicative plasmid pSOKA and integrative plasmid pR3A. Introduction of these plasmids into S. nogalater wild type cells resulted in enhanced nogalamicin biosynthesis. A similar effect was observed at heterologous expression of gene (p)ppGpp-synthetase gene relA cloned from Streptomyces coelicolor A3(2). Heterologous expression of genes absA2 from Streptomyces ghanaensis ATCC14672 and lndYR from genome Streptomyces globisporus 1912 decreased synthesis of antibiotic. The study results indicate the presence of homologs of these genes in chromosome of S. nogalater, their possible participation in regulation of nogalamicin biosynthesis, and provide us with a possibility for genetic design of the strains with higher synthesis of this antibiotic.
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Original Russian Text © D.A. Klimishin, M.V. Rabyk, T.P. Gren’, O.Ya. Nimets’, M.A. Gonchar, A.N. Gromyko, V.A. Fedorenko, 2011, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2011, Vol. 47, No. 6, pp. 650–654.
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Klimishin, D.A., Rabyk, M.V., Gren’, T.P. et al. Construction of Streptomyces nogalater Lv65 strains with enhanced nogalamicin biosynthesis using regulatory genes. Appl Biochem Microbiol 47, 594–598 (2011). https://doi.org/10.1134/S0003683811060068
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DOI: https://doi.org/10.1134/S0003683811060068