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Immunochromatographic technique for express determination of ampicillin in milk and dairy products

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Abstract

An immunochromatographic method for determination of β-lactam antibiotic ampicillin has been developed. The method is based on the competitive interaction between antibiotic molecules contained in the sample and protein conjugate of penicillin immobilized on a membrane for binding with specific antibodies labeled with colloidal gold, which occurs during movement of the sample to be tested and reagents along the membrane. The completion of the test system ensures control of exceeding the maximum permissible content of the antibiotic in milk and dairy products (10 ng/mL). The possibility of testing milk, raw milk, and dairy products for 10 minutes at room temperature without sample pretreatment has been demonstrated.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia

    N. A. Byzova, E. A. Zvereva, A. V. Zherdev & B. B. Dzantiev

Authors
  1. N. A. Byzova
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  2. E. A. Zvereva
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  3. A. V. Zherdev
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  4. B. B. Dzantiev
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Correspondence to N. A. Byzova.

Additional information

Original Russian Text © N.A. Byzova, E.A. Zvereva, A.V. Zherdev, B.B. Dzantiev, 2011, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2011, Vol. 47, No. 6, pp. 685–693.

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Byzova, N.A., Zvereva, E.A., Zherdev, A.V. et al. Immunochromatographic technique for express determination of ampicillin in milk and dairy products. Appl Biochem Microbiol 47, 627–634 (2011). https://doi.org/10.1134/S0003683811060032

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  • DOI: https://doi.org/10.1134/S0003683811060032

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