Abstract
Using the recombinant flocculating Angel yeast F6, long-term repeated batch fermentation for ethanol production was performed and a high volumetric productivity resulted from half cells not washed and the optimum opportunity of residual glucose 20 g l−1 of last medium. The obtained highest productivity was 2.07 g l−1 h−1, which was improved by 75.4% compared with that of 1.18 g l−1 h−1 in the first batch fermentation. The ethanol concentration reached 8.4% corresponding to the yield of 0.46 g g−1. These results will contribute greatly to the industrial production of fuel ethanol using the commercial method with the flocculating yeast.
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Galbe, M. and Zacchi, G., Appl. Microbiol. Biotechnol., 2002, vol. 59, pp. 618–628.
Brandberg, T., Gustafsson, L., and Franzen, C. J., Enzyme Microbiol. Technol., 2007, vol. 40, pp. 585–593.
Albers, E., Larsson, C, Liden, G., Niklasson, C, and Gustafsson, G., Appl. Micribiol. Biotechnol., 1996, vol. 62, pp. 3187–95.
Oura, E., Process Biochem., 1977, vol. 249, pp. 19–35.
Verduyn, C, Postma, E., Scheffers, W.A., and van Dijken, J.F., J. Gen. Microbiol, 1990, vol. 136, pp. 395–403.
Nissen, T.L., Kielland-Brandt, M.C., Nielsen, J., and Villadsen, J., Metabolic Engineering, 2000, vol. 2, pp. 69–77.
Blomberg, A. and Adler, L. Adv. Microb. Physiol., 1992, vol. 33, pp. 145–212.
Wang, F.Z., Appl. Biochem. Microbiol., 2009, vol. 45, no. 5, pp. 586–591.
Remize, F., Schorr-Galindo, S., Guiraud, J.R, Dequin, S., and Blondin, B., Biotechnol. Lett., 1998, vol. 20, pp. 313–318.
Watari, J., Nomura, M., Sahara, H., Koshino, S., and Keranen, S., J. Inst. Brew., 1994, vol. 100, pp. 73–77.
Verstrepen, K.J., Bauer, F.F., Michiels, C., Derdelinckx, G., Delvaux, F.R., and Pretorius, I.S., Eur. Brew. Conv. Monogr., 1999, vol. 28, pp. 30–42.
Verstrepen, K.J., Michiels, C, Derdelinckx, G., Delvaux, F.R., Winderickx, J., Thevelein, J.M., Bauer, F.F., and Pretorius, I.S., J. Am. Soc. Brew. Chem., 2001, vol. 59, pp. 69–76.
Liu, Y.Q., and Liu., D.H., Proc. Biochem., 2004, vol. 39, pp. 1507–1510.
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Wang, F.Z., Xie, T. & Hui, M. Increase of ethanol productivity by cell-recycle fermentation of flocculating yeast. Appl Biochem Microbiol 47, 527–531 (2011). https://doi.org/10.1134/S0003683811050164
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DOI: https://doi.org/10.1134/S0003683811050164