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Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria

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An Errata to this article was published on 29 December 2011

Abstract

Synthesis of 3- and 4-hydroxybutyrate copolymer (3HB-co-4HB), the most promising member of the biodegradable polyhydroxyalkanoate (PHA) family, has been studied. Cultivation conditions of naturally occurring strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus B10646 have been optimized to ensure efficient synthesis of the 3HB-co-4HB copolymer. A set of highly pure samples of the 3HB-co-4HB copolymer with 4HB content varying from 8.7 to 24.3 mol% has been obtained. Incorporation of 4HB into the copolymer was shown to cause a more pronounced decrease in polymer crystallinity than the incorporation of 3-hydroxyvalerate or 3-hydroxyhexanoate; samples with a degree of crystallinity below 30% have been obtained. The weight average molecular mass of the 3HB-co-4HB copolymers was shown to be independent on the monomer ratio and to vary broadly (from 540 to 1110 kDa).

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Authors and Affiliations

  1. Siberian Federal University, Krasnoyarsk, 660041, Russia

    T. G. Volova

  2. Biophysics Institute, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russia

    N. O. Zhila & G. S. Kalacheva

  3. Chemistry and Chemical Technology Institute, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russia

    V. A. Sokolenko

  4. Massachusetts Institute of Technology, Cambridge, MA, 02139, USA

    E. J. Sinski

Authors
  1. T. G. Volova
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  2. N. O. Zhila
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  3. G. S. Kalacheva
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  4. V. A. Sokolenko
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  5. E. J. Sinski
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Correspondence to T. G. Volova.

Additional information

Original Russian Text © T.G. Volova, N.O. Zhila, G.S. Kalacheva, V.A. Sokolenko, E.J. Sinski, 2011, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2011, Vol. 47, No. 5, pp. 544–550.

An erratum to this article can be found at http://dx.doi.org/10.1134/S000368381215001X

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Volova, T.G., Zhila, N.O., Kalacheva, G.S. et al. Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria. Appl Biochem Microbiol 47, 494–499 (2011). https://doi.org/10.1134/S0003683811050152

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  • DOI: https://doi.org/10.1134/S0003683811050152

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