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Obtaining of water soluble microbial melanin and study of its some properties

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Abstract

The effective sorption method for melanin isolation and purification from fermentation solutions of Bacillus thuringiensis serovar galleriae K1 has been elaborated, the principle process flowsheet is presented. The identification of obtained pigment with the samples of natural and synthetic melanin was done by IR-spectroscopy, and the intensity ratio of optical absorption at 650 and 500 nm allows to refer the isolated melanin to eumelanin class. By thermal treatment it was determined, that melanin’s amorphous sediment is steady at up to 120°C) the concentration of paramagnetic centers being changed from 0.053 × 1018 spin/g (48°C) to 0.25 × 1018 spin/g (120°C). The rising of the temperature of treatment up to 210°C brings to substantial increase of the concentration of unpaired electrons, and at 280°C its sharp growth is observed. At 350°C growth stops, then sharp decrease is observed. The obtained results were confirmed by methods of IR spectroscopy and derivatographic analysis.

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Authors and Affiliations

  1. SPC “Armbiotechnology” NAS RA 14 Gyurjyan Str., Yerevan, 0056, Armenia

    A. A. Aghajanyan, R. A. Asaturian, A. A. Hambardzumyan, L. B. Sargsyan, A. S. Hovsepyan, A. H. Vardanyan & A. S. Saghyan

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  1. A. A. Aghajanyan
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  2. R. A. Asaturian
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  3. A. A. Hambardzumyan
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  4. L. B. Sargsyan
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  5. A. S. Hovsepyan
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  6. A. H. Vardanyan
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  7. A. S. Saghyan
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Correspondence to A. A. Aghajanyan.

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Original Russian Text © A.A. Aghajanyan, R.A. Asaturian, A.A. Hambardzumyan, L.B. Sargsyan, A.S. Hovsepyan, A.H. Vardanyan, A.S. Saghyan, 2011, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2011, Vol. 47, No. 5, pp. 551–557.

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Aghajanyan, A.A., Asaturian, R.A., Hambardzumyan, A.A. et al. Obtaining of water soluble microbial melanin and study of its some properties. Appl Biochem Microbiol 47, 500–506 (2011). https://doi.org/10.1134/S0003683811050024

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  • DOI: https://doi.org/10.1134/S0003683811050024

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