Isolation and characterization of antibiotics produced by Streptomyces chrysomallus R-21 and S. globisporus L-242 strains

Abstract

Chrysomal and globerin complexes containing antibiotics from polyether and heptaene aromatic macrolide groups have been isolated from the mycelium of Streptomyces chrysomallus R-21 and Streptomyces globisporus L-242 strains, respectively. Physicochemical characteristics of these complexes have been investigated, including UV and NMR spectra, chromatographic parameters, etc. The fungicidal and antiviral activity of the studied complexes has been demonstrated on tomato plants. The obtained data allow us to consider the above-mentioned antibiotics to be original preparations.

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Abbreviations

a.m.u.:

atomic mass unit

TMV:

tobacco mosaic virus

HPLC:

high-performance liquid chromatography

HMS:

hexamethyl silane

DMSO:

dimethyl sulfoxide

DMFA:

dimethyl formamide

ppm:

parts per million

TLC:

thin-layer chromatography

BuOH:

butanol

EtOH:

ethanol

MeOH:

methanol

b.p.:

bottom phase

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Correspondence to I. I. Novikova.

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Original Russian Text © Yu.D. Shenin, I.I. Novikova, P.V. Suika Uarkaja, 2010, published in Biotekhnologiya, 2010, No. 2, pp. 41–53.

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Shenin, Y.D., Novikova, I.I. & Suika Uarkaja, P.V. Isolation and characterization of antibiotics produced by Streptomyces chrysomallus R-21 and S. globisporus L-242 strains. Appl Biochem Microbiol 46, 854–864 (2010). https://doi.org/10.1134/S0003683810090073

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Key words

  • antibiotics
  • isolation
  • identification
  • model experiment
  • polyene
  • polyether
  • streptomycete
  • fungicidal activity