Advertisement

Applied Biochemistry and Microbiology

, Volume 46, Issue 9, pp 854–864 | Cite as

Isolation and characterization of antibiotics produced by Streptomyces chrysomallus R-21 and S. globisporus L-242 strains

  • Yu. D. Shenin
  • I. I. NovikovaEmail author
  • P. V. Suika Uarkaja
Article

Abstract

Chrysomal and globerin complexes containing antibiotics from polyether and heptaene aromatic macrolide groups have been isolated from the mycelium of Streptomyces chrysomallus R-21 and Streptomyces globisporus L-242 strains, respectively. Physicochemical characteristics of these complexes have been investigated, including UV and NMR spectra, chromatographic parameters, etc. The fungicidal and antiviral activity of the studied complexes has been demonstrated on tomato plants. The obtained data allow us to consider the above-mentioned antibiotics to be original preparations.

Key words

antibiotics isolation identification model experiment polyene polyether streptomycete fungicidal activity 

Abbreviations

a.m.u.

atomic mass unit

TMV

tobacco mosaic virus

HPLC

high-performance liquid chromatography

HMS

hexamethyl silane

DMSO

dimethyl sulfoxide

DMFA

dimethyl formamide

ppm

parts per million

TLC

thin-layer chromatography

BuOH

butanol

EtOH

ethanol

MeOH

methanol

b.p.

bottom phase

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Novikova, I.I., Boikova, I.V., and Shenin, Yu.D., Vestn. Zashchity Rast., 2006, no. 3, pp. 13–21.Google Scholar
  2. 2.
    Novikova, I.I. and Shenin, Yu.D., in Mat. II Vseross. S”ezda po zashchite rastenii (Proc. II All-Russia Congr. on Plant Protection), St. Petersburg: Izd. VIZR, 2005.Google Scholar
  3. 3.
    Novikova, I.I., Shenin, Yu.D., Tsyplenkov, A.E., Fominykh, T.S., Suika, P.V., and Boykova, I.V., Vestn. Zaschity Rast., 2009, no. 2, pp. 3–19.Google Scholar
  4. 4.
    Dutcher, Y.D., Walter, D.R., and Wintersteinre, O., J. Org. Chem., 1963, vol. 28, pp. 995–998.CrossRefGoogle Scholar
  5. 5.
    Westley, G.W., Adv. Appl. Microbiol., 1977, vol. 22, pp. 177–223.CrossRefPubMedGoogle Scholar
  6. 6.
    Kazakabe, Y., Takahashi, N., Iwagaya, Y., and Seino, A., J. Antibiot., 1987, vol. 40, pp. 237–238.Google Scholar
  7. 7.
    Keller-Guslen, C., King, H.D., Ku, Z.L., and Von Wartburg, A., J. Antibiot., 1975, vol. 28, pp. 854–859.Google Scholar
  8. 8.
    Imada, A., Nozaki, Y., Hasegana, T., Mizuta, E., Igarasi, S., and Yoneda, M., J. Antibiot., 1978, vol. 31, pp. 7–14.PubMedGoogle Scholar
  9. 9.
    Dirlom, G.P., Presseau-Linabury, L., and Koss, D.A., J. Antibiot., 1990, vol. 43, pp. 127–130.Google Scholar
  10. 10.
    Tsuji, N., Terni, K., Nagashima, K., Johnson, F., and Kobayashi, M., J. Antibiot., 1980, vol. 43, pp. 94–97.Google Scholar
  11. 11.
    Anteemis, N.G.O., Omura, S., and Tanaka, H., Bull. Soc. Chem. Biol., 1977, pp. 353–356.Google Scholar
  12. 12.
    Vetlugina, L.A. and Nikitina, E.T., Protivogribkovye polienovye antibiotiki (Fungicidal Polyene Antibiotics), Alma-Ata: Nauka, 1980.Google Scholar
  13. 13.
    Shenin, Yu.D. and Belakhov, V.V., Khimiya polienovykh makrolidnykh antibiotikov (Chemistry of Polyene Macrolide Antibiotics), Moscow: VNIISENTI Minmedproma SSSR, 1989.Google Scholar
  14. 14.
    Omura, S. and Tanaka, H., in Macrolide Antibiotics: Chemistry, Biology and Practice, New York: Boca Ration Florida Academic Press, 1984, pp. 351–404.Google Scholar
  15. 15.
    Mechlinski, W. and Schaffiner, C.P., J. Chromatogr., 1974, vol. 99, pp. 619–633.CrossRefPubMedGoogle Scholar
  16. 16.
    Shenin, Yu.D. and Khimich, G.N., Antibiot. Khimioter., 1993, no. 38, pp. 19–29.Google Scholar
  17. 17.
    Mechlinski, W. and Schaffner, C.P., J. Antibiot., 1980, vol. 33, pp. 591–599.PubMedGoogle Scholar
  18. 18.
    Helbor, P., Thomson, M., and Hansen, S.H., J. Chromatogr., 1980, vol. 189, pp. 249–284.CrossRefGoogle Scholar
  19. 19.
    Hansen, S.H. and Thomson, M., J. Chromatogr., 1976, vol. 123, pp. 205–211.CrossRefPubMedGoogle Scholar
  20. 20.
    Rokatikainen, O., J. Chromatogr., 1991, vol. 588, pp. 356–360.CrossRefGoogle Scholar
  21. 21.
    Nefelova, M.V. and Pozmogova, I.N., Mikrobiologiya, 1960, vol. 29, pp. 857–861.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • Yu. D. Shenin
    • 1
  • I. I. Novikova
    • 1
    Email author
  • P. V. Suika Uarkaja
    • 2
  1. 1.All-Russia Research Institute of Plant ProtectionRussian Academy of Agricultural SciencesPushkin, St. PetersburgRussia
  2. 2.St. Petersburg State Institute of Technology (Technical University)St. PetersburgRussia

Personalised recommendations