Advertisement

Applied Biochemistry and Microbiology

, Volume 45, Issue 5, pp 494–497 | Cite as

Amine neuromediators, their precursors, and oxidation products in the culture of Escherichia coli K-12

  • V. A. Shishov
  • T. A. Kirovskaya
  • V. S. Kudrin
  • A. V. OleskinEmail author
Article

Abstract

The growth dynamics of the synthesis of monoamine neuromediators serotonin, norepinephrine, and dopamine in Escherichia coli K-12 was investigated for the first time using high performance liquid chromatography with electrodetection. Maximum (micromolar) concentrations of these compounds were detected in E. coli cells during the early growth phases; their intracellular content decreases after the transition to late growth phases. E. coli biomass contains (i) the substances DOPA and 5-hydroxytryptamine that serve in animal cells as neuromediator precursors and (ii) the products of their oxidative deamination. Presumably, the biosynthesis and degradation of monoamine neuromediators in bacterial cells involves enzyme systems analogous to those typical of animals. The culture fluid of E. coli contains micromolar concentrations of DOPA and nanomolar of serotonin, dopamine, and norepinephrine during the late growth phase. These concentrations are sufficient for animal/human receptors to bind them. This article deals with the potential biotechnological applications of the data obtained.

Keywords

Apply Biochemistry Dopa Biogenic Amine High Performance Liquid Chro Early Growth Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DOPA

dihydroxyphenylalanine

HVA

homovaline acid

5-HIAA

5-hydroxyindoleacetic acid

5-HT

serotonin (5-hydroxytryptamine)

DHPAA

dihydroxyphenylacetic acid

CFS

culture fluid supernatant

NE

norepinephrine

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Roshchina, V.V., Biomediatory v rasteniyakh. Atsetilkholin i biogennye aminy (Biomediators in Plants: Acetylcholine and Biogenic Amines), Pushchino: PNTs AN SSSR, 1991.Google Scholar
  2. 2.
    Hsu, S.C., Johansson, K.R., and Donahne, M.J., J. Parasitol., 1986, vol. 72, pp. 545–549.PubMedCrossRefGoogle Scholar
  3. 3.
    Tsavkelova, E.A., Botvinko, I.B., Kudrin, V.S., and Oleskin, A.V., Dokl. Akad. Nauk, 2000, vol. 372, pp. 840–842.Google Scholar
  4. 4.
    Kudrin, V.S., Miroshnichenko, I.I., and Raevskii, K.S., Neirokhimiya, 1988, vol. 7, no. 1, pp. 3–8.Google Scholar
  5. 5.
    Ashmarin, I.P. and Vorob’ev, A.A., Statisticheskie metody v mikrobiologicheskikh issledovaniyakh (Statistical Methods in Microbiological Studies), Leningrad: Gos. Izd. Med. Lit., 1962.Google Scholar
  6. 6.
    Oleskin, A.V., Kirovskaya, T.A., Botvinko, I.V., and Lysak, L.V., Mikrobiologiya, 1998, vol. 67, no. 3, pp. 305–312.Google Scholar
  7. 7.
    Oleskin, A.V., Botvinko, I.V., and Kirovskaya, T.A., Vestn. Mosk. Univ., Ser. Biol.., 1998, no. 4, pp. 3–10.Google Scholar
  8. 8.
    Anuchin, A.M., Chuvelev, D.I., Kirovskaya, T.A., and Oleskin, A.V., Mikrobiologiya, 2008, vol. 77, no. 6, pp. 1–8.Google Scholar
  9. 9.
    Seidakhmetova, Z.Zh. and Tashenova, G.K., Byull. SO RAMN, 2005, vol. 118, no. 4, pp. 93–95.Google Scholar
  10. 10.
    Shenderov, B.A., Meditsinskaya mikrobnaya ekologiya i funktsional’noe pitanie (Medical Microbial Ecology and Functional Nutrition), Moscow: Grant, 2001, vol. 3.Google Scholar
  11. 11.
    Dubynin, V.A., Kamenskii, A.A., Sapin, M.R., and Sivoglazov, V.I., Regulyatornye sistemy organizma cheloveka (Regulatory Systems of Human Body), Moscow: Drofa, 2003.Google Scholar
  12. 12.
    Rahn, O., Zbl. Bakteriol. Parasitenk., 1906, vol. 16, pp. 417–429.Google Scholar
  13. 13.
    Penfold, W.J., J. Hygiene, 1914, vol. 14, pp. 215–241.Google Scholar
  14. 14.
    Vakhitov, T.Ya. and Petrov, L.N., Mikrobiologiya, 2006, vol. 75, no. 4, pp. 483–488.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • V. A. Shishov
    • 1
  • T. A. Kirovskaya
    • 1
  • V. S. Kudrin
    • 2
  • A. V. Oleskin
    • 1
    Email author
  1. 1.Biology FacultyMoscow State UniversityMoscowRussia
  2. 2.Institute of PharmacologyRussian Academy of Medical SciencesMoscowRussia

Personalised recommendations