Abstract
This article describes the optimal conditions for the enzymatic hydrolysis of chitosan and its chemically-modified derivatives using the preparation extracted from the king crab hepatopancrease possessing pronounced hydrolythic activity. The following preparations were used: chitosan with a molecular weight of 700 kDa and an acetylation level of 0.15, carboxymethyl chitosan 200 kDa witih an extent of replacement of 0.23, and N-succinyl chitosan 390 kDa with an extent of replacement of 0.8. Low molecular-weight samples of chitosan and of its modified derivatives were obtained with the yields of 85, 55, and 80%, respectively. The conditions of the hydrolysis were as follows: an enzyme: substrate ratio of 1: 200, 37°C, and 20 h duration of hydrolysis.
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Original Russian Text © A.V. Ilyina, D.S. Zagorskaya, A.N. Levov, A.I. Albulov, N.P. Kovacheva, V.P. Varlamov, 2009, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2009, Vol. 45, No. 4, pp. 415–421.
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Ilyina, A.V., Zagorskaya, D.S., Levov, A.N. et al. The use of enzymatic preparation for the production of low molecular-weight chitosan from the king crab hepatopancrease. Appl Biochem Microbiol 45, 374–379 (2009). https://doi.org/10.1134/S0003683809040048
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DOI: https://doi.org/10.1134/S0003683809040048