Abstract
Four monoclonal antibodies (mAbs), G6, F9, H8, and B2, against human alpha-1-microglobulin (A1M) have been produced and characterized. The parameters of affinity (Kp ∼ 109 M−1), epitope specificity (the additively binding G6/F9, G6/H8, G6/B2, F9/H8, and F9/B2 pairs), and the observed effect of reversibility of structural changes induced by chemical agents allow use of these mAbs in biospecific methods of A1M purification and quantitative determination. The application of mAbs to an A1M enzyme immunoassay (analytical sensitivity—0.5 μg/l) and one step isolation of pure A1M by immunoaffinity chromatography was described.
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Akerstrom, B., Logdberg, L., Berggard, T., Osmark, P., and Lindqvist, A., Biochim. Biophys. Acta, 2000, vol. 1482, nos. 1–2, pp. 172–184.
Ekstrom, B. and Berggard, I., J. Biol. Chem., 1977, vol. 252, no. 1, pp. 8048–8051.
Flower, D.R., Biochem. J., 1996, vol. 318, no. 1, pp. 1–14.
Akerstrom, B., Flower, D.R., and Salier, J.-P., Biochim. Biophys. Acta, 2000, vol. 1482, nos. 1–2, pp. 1–8.
Amoresano, A., Minchiotti, L., Cosulich, M.E., Campagnoli, M., Pucci, P., Andolfo, A., Gianazza, E., and Galliano, M., Eur. J. Biochem., 2000, vol. 267, no. 7, pp. 2105–2112.
Escribano, J., Grubb, A., Calero, M., and Mendez, E., J. Biol. Chem., 1991, vol. 266, no. 24, pp. 15758–15763.
Berggard, T., Cohen, A., Persson, P., Lindquist, A., Cedervall, T., Silow, M., Thogersen, I.B., Jonsson, J.A., Enghild, J.J., and Akerstrom, B., Protein Sci., 1999, vol. 8, no. 12, pp. 2611–2620.
Sala, A., Campagnoli, M., Perani, E., Romano, A., Labo, S., Monzani, E., Minchiotti, L., and Galliano, M., J. Biol. Chem., 2004, vol. 279, no. 49, pp. 51033–51041.
Logdberg, L. and Akerstrom, B., Scand. J. Immunol., 1981, vol. 13, no. 4, pp. 383–390.
Allhorn, M., Berggard, T., Nordberg, J., Olsson, M.L., and Akerstrom, B., Blood, 2002, vol. 99, no. 6, pp. 1894–1901.
Larsson, J., Allhorn, M., and Akerstrim, B., Arch. Biochem. Biophys., 2004, vol. 432, no. 2, pp. 196–204.
Aloven, T., Jarup, L., and Elinder, C.-G., Environ. Health Perspect., 2002, vol. 110, no. 7, pp. 699–702.
Hong, C.Y., Hughes, K., Chia, K.S., and Ling, S.L., Diabetes Care, 2003, vol. 26, no. 2, pp. 338–342.
Niederstadt, C., Happ, T., Tatsis, E., Schnabel, A., and Steinhoff, J., Rheumatology, 1999, vol. 38, no. 1, pp. 28–33.
Penders, J. and Delanghe, J.R., Clin. Chim. Acta, 2004, vol. 346, no. 2, pp. 107–118.
Sear, J.W., Brit. J. Anaesth., 2005, vol. 95, no. 1, pp. 20–32.
Serchenya, T.S., in Sbornik trudov molodykh uchenykh NAN Belarusi. Materialy Mezhd. nauchn. konf (Collected Papers of Young Scientists of NAN of Belarus, Proc. Int. Sci. Conf.), Minsk: IP Loginov, 2004, vol. 2, pp. 67–71.
Serchenya, T.S., Drozhdenyuk, A.P., Pryadko, A.G., Vashkevich, I.I., and Sviridov, O.V., Vestsi NAN Belarusi, Ser. Khim., 2006, no. 2, pp. 68–72.
Steubach, M. and Aurdan, R., Arch. Biochem. Biophys., 1969, vol. 134, no. 2, pp. 270–284.
Mirrales, F., Takeda, Y., and Escribano, M.J., J. Immunol. Methods, 1991, vol. 140, no. 2, pp. 191–196.
Antitela. Metody: Kn. 1 (Antibodies: Methods. Book 1), Ketty, D., Ed., Moscow: Mir, 1991.
Friquet, B., Djavadi-Ohaniance, I., Pages, J., Bussard, A., and Goldberg, M., J. Immunol. Methods, 1983, vol. 60, no. 3, pp. 351–358.
Hurn, B.A.L. and Chanter, S.M., Methods Enzymol., 1980, vol. 70, no. 1, pp. 104–142.
Turkova, J., Affinity Chromatography, New York: Elsevier, 1978. Translated under the title Affinnaya khromatografiya, Moscow: Mir, 1980.
Subramanian, A., Mol. Biotechnol., 2002, vol. 20, no. 1, pp. 41–47.
Jack, G.W., Mol. Biotechnol, 1994, vol. 1, no. 1, pp. 59–86.
Kamihira, M., Iijima, S., and Kobayashi, T., Bioseparation, 1992, vol. 3, nos. 2–3, pp. 185–188.
Fernandez-Luna, J.L., Moneo, I., Grubb, A., and Mendez, E., J. Immunol. Methods, 1985, vol. 82, no. 1, pp. 101–110.
Vincent, C. and Revillard, J.P., J. Immunol. Methods, 1985, vol. 82, no. 1, pp. 111–119.
Itoh, Y., Nishino, H., and Enomoto, H., Clin. Chim. Acta, 1986, vol. 155, no. 3, pp. 221–226.
Porstmann, T., Schmechta, H., Hentschel, Ch., Doepel, H., Pas, P., Becker, J., Pergande, M., Jung, K., and Nugel, E., J. Clin. Chem. Clin. Biochem, 1990, vol. 28, no. 10, pp. 669–675.
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Original Russian Text © T.S. Serchenya, A.G. Pryadko, O.V.Sviridov, 2009, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2009, Vol. 45, No. 3, pp. 361–368.
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Serchenya, T.S., Pryadko, A.G. & Sviridov, O.V. Antigen-binding activity, structural characteristics and use of monoclonal antibodies to human alpha-1-microglobulin. Appl Biochem Microbiol 45, 326–333 (2009). https://doi.org/10.1134/S0003683809030156
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DOI: https://doi.org/10.1134/S0003683809030156