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The influence of conditions of Acinetobacter calcoaceticus K-4 strain cultivation on surface-active substances synthesis

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Abstract

It has been observed that the Acinetobacter calcoaceticus K-4 strain produces surface-active substances (SAS) while growing either on hydrophilic (ethanol) or on hydrophobic substrates (hexadecane). Maximal SAS synthesis (with a conditional SAS concentration of 3.6; emulsifying activity of culture liquid dissolved in 50 times equal to 96%) was detected with growth on an ethanol-containing medium with the addition of urea, yeast autolysate and microelements, C/N ratio 60:1 and 10% inoculate, cultivated on ethanol-containing medium by the end of the exponential phase of growth. With respect to its chemical nature, extracellular SAS synthesized by A. calcoaceticus K-4 growing on ethanol-containing medium under optimal cultivating conditions form a glycolipid-aminolipid complex.

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Authors and Affiliations

  1. National University of Food Technologies, 010033, Kiev, Ukraine

    T. P. Pirog & S. I. Antonyuk

  2. Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 03680, Kiev, Ukraine

    T. A. Shevchuk

  3. Department of Physical Chemistry of Fossil Fuels, Institute of Physical Organic Chemistry and Coal Chemistry, National Academy of Sciences of Ukraine, 79060, Lvov, Ukraine

    Ye. V. Karpenko

Authors
  1. T. P. Pirog
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  2. S. I. Antonyuk
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  3. Ye. V. Karpenko
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  4. T. A. Shevchuk
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Correspondence to T. P. Pirog.

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Original Russian Text © T.P. Pirog, S.I. Antonyuk, Ye.V. Karpenko, T.A. Shevchuk, 2009, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2009, Vol. 45, No. 3, pp. 304–310.

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Pirog, T.P., Antonyuk, S.I., Karpenko, Y.V. et al. The influence of conditions of Acinetobacter calcoaceticus K-4 strain cultivation on surface-active substances synthesis. Appl Biochem Microbiol 45, 272–278 (2009). https://doi.org/10.1134/S0003683809030065

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  • DOI: https://doi.org/10.1134/S0003683809030065

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