Antioxidant properties of essential oils from lemon, grapefruit, coriander, clove, and their mixtures


Antioxidant properties of individual essential oils from lemon (Citrus limon L.), pink grapefruit (Citrus paradisi L.), coriander (Coriandrum sativum L.), and clove (Caryophyllus aromaticus L.) buds and their mixtures were studied by capillary gas-liquid chromatography. Antioxidant activity was assessed by oxidation of the aliphatic aldehyde hexanal to the carboxylic acid. The lowest and highest antioxidant activities were exhibited by grapefruit and clove bud essential oils, respectively. Mixtures containing clove bud essential oil also strongly inhibited oxidation of hexanal. Changes in the composition of essential oils and their mixtures in the course of long-term storage in the light were studied. The stability of components of lemon and coriander essential oils in mixtures increased compared to individual essential oils.

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  1. 1.

    Madsen, L.H., Nielsen, B.R., Bertelsen, G., and Skibsted, L.H., Food Chem., 1996, vol. 57, pp. 331–337.

    Article  CAS  Google Scholar 

  2. 2.

    Voitkevich, S.A., Efirnye masla dlya parfyumerii i aromaterapii (Essential Oils for Perfumery and Aromatherapy), Moscow: Pishch. promyshlennost’, 1999.

    Google Scholar 

  3. 3.

    Lee, K.G. and Shibamoto, T., J. Agric. Food Chem., 2002, vol. 50, no. 15, pp. 4947–4952.

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Huang, D., Ou, B., and Prior, R.L., J. Agric. Food Chem., 2005, vol. 53, no. 3, pp. 1841–1846.

    PubMed  Article  CAS  Google Scholar 

  5. 5.

    Cervato, G., Carabelli, M., Gervasio, S., Cittera, A., Cazzola, R., and Cestaro, B., J. Food Biochem., 2000, vol. 24, no. 3, pp. 453–465.

    Article  CAS  Google Scholar 

  6. 6.

    Dorman, H.J.D., Figueiredo, A.C., Barroso, J.G., and Deans, S.G., Flavour Fragr. J., 2000, vol. 15, pp. 12–15.

    Article  CAS  Google Scholar 

  7. 7.

    Dang, M.N., Takascova, M., Nguyen, D.V., and Kristianova, K., Nahrung, 2001, vol. 45, no. 1, pp. 64–66.

    PubMed  Article  CAS  Google Scholar 

  8. 8.

    Lee, K.G. and Shibamoto, T., Food Chem., 2001, vol. 74, pp. 443–448.

    Article  CAS  Google Scholar 

  9. 9.

    Lee, K.W., Kim, Y.J., Kim, D.-O., Lee, H.J., and Lee, C.Y., J. Agric. Food Chem., 2003, vol. 51, no. 22, pp. 6516–6520.

    PubMed  Article  CAS  Google Scholar 

  10. 10.

    Kahkonen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kujala, T.S., and Heinonen, I.M., J. Agric. Food Chem., 1999, vol. 47, no. 8, pp. 3954–3962.

    PubMed  Article  CAS  Google Scholar 

  11. 11.

    Areias, F., Valentao, P., Andrade, P.B., Ferreres, F., and Seabra, R.M., J. Agric. Food Chem., 2000, vol. 48, no. 13, pp. 6081–6084.

    PubMed  Article  CAS  Google Scholar 

  12. 12.

    Ruberto, G. and Baratta, M., Food Chem., 2002, vol. 69, pp. 167–174.

    Article  Google Scholar 

  13. 13.

    Yanishlieva, N.V., Mariniva, E.M., Gordon, M.H., and Raneva, V.G., Food Chem., 1999, vol. 64, pp. 59–66.

    Article  CAS  Google Scholar 

  14. 14.

    Bowry, V.W. and Ingold, K.U., Acc. Chem. Res., 1999, vol. 32, no. 1, pp. 27–34.

    Article  CAS  Google Scholar 

  15. 15.

    Foti, M.C. and Ingold, K.U., J. Agric. Food Chem., 2003, vol. 51, no. 9, pp. 2758–2765.

    PubMed  Article  CAS  Google Scholar 

  16. 16.

    Sivropoulou, A., Papanikolaou, E., Nikolaou, C., Kokkini, S., Lanaras, T., and Arsenakis, M., J. Agric. Food Chem., 1996, vol. 44, no. 5, pp. 1202–1205.

    Article  CAS  Google Scholar 

  17. 17.

    Misharina, T.A., Golovnya, R.V., and Beletskii, I.V., Zh. Analit. Khim., 1999, vol. 54, no. 2, pp. 219–222.

    Google Scholar 

  18. 18.

    Gopolakrishnan, N., J. Agric. Food Chem., 1994, vol. 42, no. 3, pp. 796–798.

    Article  Google Scholar 

  19. 19.

    Misharina, T.A., Prikl. Biokhim. Mikrobiol., 2001, vol. 37, no. 6, pp. 726–732.

    PubMed  CAS  Google Scholar 

  20. 20.

    Misharina, T.A., Polshkov, A.N., Ruchkina, E.L., and Medvedeva, I.B., Prikl. Biokhim. Mikrobiol., 2003, vol. 39, no. 3, pp. 353–358.

    PubMed  CAS  Google Scholar 

  21. 21.

    Misharina, T.A. and Polshkov, A.N., Prikl. Biokhim. Mikrobiol., 2005, vol. 41, no. 6, pp. 693–702.

    PubMed  CAS  Google Scholar 

  22. 22.

    Jennings, W. and Shibamoto, T., Qualitative Analysis of the Flavor and Fragrance Volatiles by Glass Capillary Gas Chromatography, New York: Academic, 1980, pp. 130–154.

    Google Scholar 

  23. 23.

    Lee, S.J., Shibamoto, T., and Lee, K.G., Food Chem., 2005, vol. 91, pp. 131–137.

    Article  CAS  Google Scholar 

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Correspondence to T. A. Misharina.

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Original Russian Text © T.A. Misharina, A.L. Samusenko, 2008, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2008, Vol. 44, No. 4, pp. 482–486.

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Misharina, T.A., Samusenko, A.L. Antioxidant properties of essential oils from lemon, grapefruit, coriander, clove, and their mixtures. Appl Biochem Microbiol 44, 438–442 (2008).

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  • Antioxidant Activity
  • Apply Biochemistry
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