Skip to main content
SpringerLink
Log in

Use of soybean peroxidase for the enzyme immunoassay of sulfamethoxipyridazine in milk

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

An enzyme immunoassay with colorimetric detection of sulfamethoxipyridazine (SMP), the most widely used sulfamide, was developed with the soybean anionic peroxidase as an enzyme marker. The range of SMP detection is 1.3–63.0 ng/ml with a detection limit of 0.4 ng/ml. The root square deviation of detection results did not exceed 6%. It was demonstrated that 0.15% casein added to the working buffer prevented the effect of the milk matrix on the detection. The results obtained demonstrate that the assay developed is promising, displaying a sensitivity that exceeds the maximum permissible concentration of sulfamides in milk (100 μg/l) by several orders of magnitude.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bach, A. and Chodat, R., Ber. Deutsch. Chem. Gesell., 1903, vol. 36, pp. 600–605.

    Article  Google Scholar 

  2. Sakharov, I.Yu., Biokhimiya, 2004, vol. 69, no. 8, pp. 1013–1020.

    Google Scholar 

  3. Gazaryan, I.G., Khushpul’yan, D.M., and Tishkov, V.I., Usp. Biol. Khim., 2006, vol. 46, pp. 303–322.

    CAS  Google Scholar 

  4. Gazaryan, I.G. and Lagrimini, L.M., Phytochemistry, 1996, vol. 41, no. 4, pp. 1029–1034.

    Article  PubMed  CAS  Google Scholar 

  5. Kvaratskhelia, M., Winkel, C., and Thorneley, R.N.F., Plant Physiol., 1997, vol. 114, no. 4, pp. 1237–1245.

    Article  PubMed  CAS  Google Scholar 

  6. Bernards, M.A., Fleming, W.D., Llewellyn, D.B., Priefer, R., Yang, X., Sabatino, A., and Plourde, G.L., Plant Physiol., 1999, vol. 121, no. 1, pp. 135–145.

    Article  PubMed  CAS  Google Scholar 

  7. Castillo, Leon J., Alpeeva, I.S., Chubar, T.A., Galaev, I.Yu., Csoregi, E., and Sakharov, I.Yu., Plant Sci., 2002, vol. 163, no. 5, pp. 1011–1019.

    Article  Google Scholar 

  8. Gillikin, J.W. and Graham, J.S., Plant Physiol., 1991, vol. 96, no. 1, pp. 214–220.

    Article  PubMed  CAS  Google Scholar 

  9. McEldoon, J.P. and Dordick, J.S., Biotechnol. Progr., 1996, vol. 12, no. 4, pp. 555–558.

    Article  CAS  Google Scholar 

  10. Ryan, B.J. and Carolan, N., Ó. Fágáin C., Trends Biotechnol., 2006, vol. 24, no. 8, pp. 355–363.

    Article  PubMed  CAS  Google Scholar 

  11. Ungemach, F.R., Acta Vet. Scand., 2000, vol. 93.

  12. Svensson, C.K., Cowen, E.W., and Gaspari, A.A., Pharm. Rev., 2000, vol. 53, no. 3, pp. 357–379.

    Google Scholar 

  13. Skold, O., Vet. Res., 2001, vol. 32, nos. 3–4, pp. 261–273.

    Article  PubMed  CAS  Google Scholar 

  14. Teuber, M., Curr. Opin. Microbiol., 2001, vol. 4, no. 5, pp. 493–499.

    Article  PubMed  CAS  Google Scholar 

  15. Sheth, H.B. and Sporns, P., J. Agric. Food Chem., 1991, vol. 39, no. 9, pp. 1696–1700.

    Article  CAS  Google Scholar 

  16. Muldoon, M.T., Font, I.A., Beier, R.C., Holtzapple, C.K., Young, C.R., and Stanker, L.H., Food Agric. Immunol., 1999, vol. 11, no. 2, pp. 117–134.

    Article  CAS  Google Scholar 

  17. Spinks, C.A., Wyatt, G.M., Lee, H.A., and Morgan, M.R.A., Bioconjugate Chem., 1999, vol. 10, no. 4, pp. 583–588.

    Article  CAS  Google Scholar 

  18. Kohen, F., Gayer, B., Amir-Zaltsman, Y., and O’Keeffe, M., Food Agric. Immunol., 2000, vol. 12, no. 3, pp. 193–201.

    Article  CAS  Google Scholar 

  19. Muldoon, M.T., Holtzapple, C.K., Deshpande, S.S., Beier, R.C., Stanker, L.H., Beier, R.C., and Stanker, L.H., J. Agric. Food Chem., 2000, vol. 48, no. 2, pp. 537–544.

    Article  PubMed  CAS  Google Scholar 

  20. Cliquet, P., Cox, E., Haasnoot, W., Schacht, E., and Gooddeeris, B.M., J. Agric. Food Chem., 2003, vol. 51, no. 20, pp. 5835–5842.

    Article  PubMed  CAS  Google Scholar 

  21. Strom, B.L., Schinnar, R., Apter, A.J., Margolis, D.J., Lautenbach, E., Hennessy, S., Bilker, W.B., and Pettitt, D., N. Engl. J. Med., 2003, vol. 349, no. 17, pp. 1628–1635.

    Article  PubMed  CAS  Google Scholar 

  22. Pastor-Navarro, N., Garcia-Bover., Maquieira A., Puchades R, Anal. Bioanal. Chem., 2004, vol. 379, nos. 7–8, pp. 1088–1099.

    PubMed  CAS  Google Scholar 

  23. Bienenmann-Ploum, M., Korpimaki, T., Haasnoot, W., and Kohen, F., Anal. Chim. Acta, 2005, vol. 529, nos. 1–2, pp. 115–122.

    Article  CAS  Google Scholar 

  24. Eremin, S.A., Murtazina, N.R., Ermolenko, D.N., Zherdev, A.V., Mart’ianov, A.A., Yazynina, E.V., Michura, I.V., Formanovsky, A.A., and Dzantiev, B.B., Anal. Lett., 2005, vol. 38, no. 6, pp. 951–969.

    CAS  Google Scholar 

  25. Ermolenko, D.N., Eremin, S.A., Mart’ianov, A.A., Zherdev, A.V., and Dzantiev, B.B., Anal. Lett., 2007, vol. 40, no. 6.

  26. Ishikawa, E., Imagawa, M., Hashida, S., Yoshitake, S., Hamaguchi, Y., and Ueno, T., J. Immunoassay, 1983, vol. 4, no. 3, pp. 209–327.

    Article  PubMed  CAS  Google Scholar 

  27. Sakharov, I.Yu., Alpeeva, I.S., and Efremov, E.E., J. Agric. Food Chem., 2006, vol. 54, no. 5, pp. 1584–1587.

    Article  PubMed  CAS  Google Scholar 

  28. Bayer, E.A. and Wilchek, M., Methods Enzymol., 1990, vol. 184, pp. 138–160.

    Article  PubMed  CAS  Google Scholar 

  29. Nakane, P.K. and Kawaoi, A., J. Histochem. Cytochem., 1974, vol. 22, no. 12, pp. 1084–1091.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plekhanov Russian Academy of Economics, Moscow, 113054, Russia

    A. N. Berlina & I. Yu. Sakharov

  2. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia

    A. V. Zherdev & B. B. Dzantiev

Authors
  1. A. N. Berlina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Zherdev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. B. Dzantiev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Yu. Sakharov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. N. Berlina.

Additional information

Original Russian Text © A.N. Berlina, A.V. Zherdev, B.B. Dzantiev, I.Yu. Sakharov, 2007, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2007, Vol. 43, No. 5, pp. 614–620.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Berlina, A.N., Zherdev, A.V., Dzantiev, B.B. et al. Use of soybean peroxidase for the enzyme immunoassay of sulfamethoxipyridazine in milk. Appl Biochem Microbiol 43, 550–555 (2007). https://doi.org/10.1134/S0003683807050080

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683807050080

Keywords

Search

Navigation