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Measurement of the content of the osmoprotectant ectoine in methylotrophic bacteria by means of normal-phase high performance liquid chromatography

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Abstract

Detection and quantitative analysis of ectoine in bacterial biomass were performed by normal-phase high performance liquid chromatography with ultraviolet detection at 230 nm. Quantitative analysis was not hindered by glutamate and sucrose accumulation in bacteria. Measurement of ectoine concentration in haloalkaliphilic methanotrophs Methylobacter marinus 7C and Methylomicrobium alcaliphilum 5S showed that ectoine accumulation reached maximum (5 and 12% of dry cell weight) in the presence of NaCl at concentrations of 4 and 6%, respectively.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290, Russia

    B. Ts. Eshinimaev & V. N. Khmelenina

  2. Pushchino State University, Pushchino, 142290, Russia

    I. S. Tsyrenzhapova & Yu. A. Trotsenko

Authors
  1. B. Ts. Eshinimaev
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  2. I. S. Tsyrenzhapova
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  3. V. N. Khmelenina
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  4. Yu. A. Trotsenko
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Original Russian Text © B.Ts. Eshinimaev, I.S. Tsyrenzhapova, V.N. Khmelenina, Yu.A. Trotsenko, 2007, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2007, Vol. 43, No. 2, pp. 215–218.

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Eshinimaev, B.T., Tsyrenzhapova, I.S., Khmelenina, V.N. et al. Measurement of the content of the osmoprotectant ectoine in methylotrophic bacteria by means of normal-phase high performance liquid chromatography. Appl Biochem Microbiol 43, 193–196 (2007). https://doi.org/10.1134/S0003683807020111

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  • DOI: https://doi.org/10.1134/S0003683807020111

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