Abstract
A regulatory protein displaying biological activity at ultralow doses was identified in cow’s milk. The isoelectric point for this protein falls into the pH range of 4.48–5.59, and the molecular weight does not exceed 10 kDa. A study of the secondary structure detected the predominant presence of β-structures, especially antiparallel, in the molecule of this regulatory protein as well as the regions described in terms of a statistical globule. It was demonstrated that this protein is located extracellularly in the epithelium of mammary ducts, and that this regulatory protein is present in an active form in whole milk; however, it was detectable neither in dry milk nor in infant formula. The results obtained suggest that the milk regulatory protein characterized in this work was identical to low-molecular-weight serum glycoprotein, one of the proteins studied earlier, displaying biological activity at ultralow doses.
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Original Russian Text © P.A. Nazarova, M.S. Krasnov, V.P. Yamskova, IA. Yamskov, 2006, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2006, Vol. 42, No. 5, pp. 529–533.
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Nazarova, P.A., Krasnov, M.S., Yamskova, V.P. et al. A regulatory protein isolated from cattle milk. Appl Biochem Microbiol 42, 464–467 (2006). https://doi.org/10.1134/S0003683806050036
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DOI: https://doi.org/10.1134/S0003683806050036